外源海藻糖对低温胁迫下玉米幼苗根系生长及生理特性的影响

doi:10.3969/j.issn.1000?6362.2018.08.006

摘要/Abstract

摘要： 以玉米品种“先玉335”为实验材料，采用盆栽培养方法，待幼苗长至三叶一心时进行根系浇注海藻糖处理，海藻糖浓度分别为3、6、9、12、15、18mmolL-1，以清水为对照，在人工智能培养箱昼/夜温度14℃/5℃的低温条件下培养4d，分析低温胁迫下海藻糖对玉米幼苗根系生长、抗氧化酶活性、细胞膜稳定性及渗透调节物质含量的影响。结果显示：根系浇注海藻糖能促进低温胁迫下玉米幼苗根系生长，诱导细胞抗氧化酶活性增加，维持根系细胞膜结构和功能的稳定。与对照相比，12mmolL-1海藻糖处理的幼苗根长、根表面积和根鲜干重分别增加32.16%、15.87%、41.14%、58.33%；SOD和POD活性分别增加44.16%和62.17%；MDA含量和相对电导率分别降低50.23%和42.53%；脯氨酸和可溶性蛋白含量分别增加81.32%和87.21%。表明根系浇注适宜浓度海藻糖可有效提高玉米幼苗的抗低温能力，通过增强根系抗氧化酶活性，减轻膜损伤，进而缓解低温胁迫对幼苗生长的抑制，且12mmolL?1的海藻糖溶液根系处理效果最佳。

关键词: 玉米, 海藻糖, 低温胁迫, 抗氧化系统, 渗透调节物质

Abstract: In order to investigate the trehalose on root growth, antioxidant enzyme activity, cell membrane stability and osmotic adjustment of maize seedlings,＂Xianyu335＂was chosen as the experimental materials by pot culture method. When the seedlings grow to three leaves, the roots were treated with trehalose at the concentration of 3, 6, 9, 12, 15, 18mmolL?1, with the treatment of spraying clear water as CK. Afterwards, the seedling are placed in artificial intelligence incubator (day/night temperature of 14℃/5℃ low temperature condition) for 4 days. The results showed that the application of trehalose effectively promoted the root growth of maize seedlings under low temperature stress, enhanced the antioxidant enzyme activities, and maintained the stability of cell membrane structure and function of root system. Compared with the control, with the application of 12mmolL?1 trehalose, the root length, root surface area and root dry weight of the seedlings increased by 32.16%, 15.87%, 41.14% and 58.33%, the activities of SOD and POD increased by 44.16% and 62.17%, the content of MDA and the relative conductivity were reduced by 50.23% and 42.53%, and the contents of proline and soluble protein were increased by 81.32% and 87.21%, respectively. The results showed that the optimum concentration of trehalose could significantly improved the low temperature resistance of maize seedlings, alleviated the inhibition of low temperature stress on seedling growth, enhanced the activity of antioxidant enzymes and reduced the membrane damage, and 12mmolL?1 trehalose solution has the best treatment effect.

Key words: Maize, Trehalose, Low temperature, Antioxidant system, Osmotic adjustment substance

刘旋，佟昊阳，田礼欣，左师宇，孙磊，曲丹阳，李丽杰，魏湜，李晶. 外源海藻糖对低温胁迫下玉米幼苗根系生长及生理特性的影响[J]. 中国农业气象, 2018, 39(08): 538-547.

LIU Xuan, TONG Hao-yang, TIAN Li-xin, ZUO Shi-yu, SUN Lei, QU Dan-yang, LI Li-jie, WEI Shi, LI Jing. Effects of Exogenous Trehalose on Growth and Physiological Characteristics of Maize Seedling Roots under Chilling Stress[J]. Chinese Journal of Agrometeorology, 2018, 39(08): 538-547.

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