中国农业气象 ›› 2021, Vol. 42 ›› Issue (12): 1009-1019.doi: 10.3969/j.issn.1000-6362.2021.12.003

• 农业生物气象栏目 • 上一篇    下一篇

螯合剂HIDS对亚适温下西葫芦幼苗的影响

武月胜,焦东,李灵芝,张伟,高清兰,常领山,张杰纯,李海平   

  1. 1.山西农业大学园艺学院,太谷 030801;2. 大同市云州区农业农村局农业技术推广中心,大同 037300;3.山西省农业机械化服务中心,太原 030031;4.大同市气象局,大同 037010;5.山东远联化工股份有限公司,济宁 272000
  • 收稿日期:2021-04-27 出版日期:2021-12-20 发布日期:2021-11-28
  • 通讯作者: 李海平,副教授,主要从事设施蔬菜栽培与生理等研究工作,E-mail:lihp0205@163.com E-mail:lihp0205@163.com
  • 作者简介:武月胜,E-mail:10137700106@qq.com
  • 基金资助:
    国家重点研发计划(2020YFD1000300)

Effect of Chelating Agent HIDS on Summer Squash Seedlings under Sub-Optimal Temperature

WU Yue-sheng, JIAO Dong, LI Ling-zhi, ZHANG Wei, GAO Qing-lan, CHANG Ling-shan, ZHANG Jie-chun, LI Hai-ping   

  1. 1.College of Horticulture, Shanxi Agricultural University, Taigu 030801, China; 2. Datong Yunzhou District Agriculture Rural Bureau Agriculture Technology Popularizing Center, Datong 037300; 3.Shanxi Agricultural Mechanization Service Center, Taiyuan 030031; 4.Datong Meteorological Bureau, Datong 037010; 5.Shandong Yuanlian Chemical Company Limited, Jining 272000
  • Received:2021-04-27 Online:2021-12-20 Published:2021-11-28

摘要: 以西葫芦品种‘农园1号’为实验材料,设置7个处理:适温处理(昼/夜25℃/16℃),浇灌1/3倍山崎黄瓜配方营养液(CK);亚适温处理(昼/夜16℃/8℃),浇灌1/3倍山崎黄瓜配方营养液(TCK);亚适温条件下分别用1/3倍山崎黄瓜配方营养液配成HIDS浓度为100、400、700、1000、1300mg·L−1的营养液浇灌(分别为T1、T2、T3、T4和T5处理),西葫芦幼苗在人工气候室内培养18d后,分析添加HIDS对西葫芦幼苗生长指标,叶片渗透调节物质、MDA、抗氧化酶活性、光合特性及荧光参数的影响。结果表明:TCK处理西葫芦幼苗生长状况、抗氧化酶活性以及光合能力均比CK处理差。亚适温下不同浓度HIDS处理均可缓解亚适温对西葫芦幼苗生长的抑制,最适浓度为700mg·L−1(T3),其单株叶面积、全株鲜重、干重、可溶性蛋白、脯氨酸含量、POD、SOD、CAT活性比TCK处理显著提高,MDA含量显著降低;叶绿素总量以及净光合速率、蒸腾速率、胞间二氧化碳浓度和气孔导度显著提高;叶片Fv/Fm、ΦPSⅡ、Fv'/Fm'、ETR、qP升高,NPQ降低,可缓解亚适温对西葫芦光合器官造成的伤害。因此,添加适宜浓度的HIDS可通过促进西葫芦幼苗生物量积累及叶片中渗透调节物质的积累,减轻细胞膜脂过氧化程度,提高抗氧化酶活性和光合能力,以此提高西葫芦幼苗对亚适温的适应能力,以HIDS浓度为700mg·L−1时各指标最接近CK处理,缓解效果最好。

关键词: 西葫芦, 亚适温, HIDS, 抗氧化性, 光合特性

Abstract: In this experiment, summer squash variety' Nongyuan No.1' was used as experimental material, and seven treatments were set up: suitable temperature treatment (day/night 25℃/16℃, CK), watering 1/3 times Yamazaki cucumber formula; Suboptimal temperature treatment (day/night 16℃/8℃, TCK), watering 1/3 times Yamazaki cucumber formula. Under sub-optimal temperature conditions, 1/3 times Yamazaki cucumber formula was used to prepare nutrient solution with HIDS concentration of 100, 400, 700, 1000, 1300mg·L−1(T1, T2, T3, T4 and T5 treatments, respectively). After 18 days of indoor culture in artificial climate, the effects of HIDS on the growth index, osmotic adjustment substances in leaves and MDA of summer squash seedlings were analyzed. The results showed that the growth status, antioxidant enzyme activity and photosynthetic capacity of summer squash treated with TCK were worse than those treated with CK. Under suboptimal temperature, HIDS treatment with different concentrations can alleviate the inhibition of suboptimal temperature on the growth of summer squash seedlings, and the optimum concentration is 700mg·L−1 (T3). Compared with TCK treatment, the leaf area per plant, fresh and dry weight of the whole plant, soluble protein, proline content, POD, SOD and CAT activities are significantly increased, and MDA content is significantly decreased. The total amount of chlorophyll, photosynthetic rate, transpiration rate, stomatal conductance increased significantly; The Fv/Fm, ΦPSⅡ, Fv'/Fm', ETR, qP and NPQ decreased, alleviate the damage caused by suboptimal temperature to photosynthetic organs of summer squash. Therefore, adding suitable concentration of HIDS can improve the adaptability of summer squash seedlings to suboptimal temperature by promoting the accumulation of biomass and osmotic adjustment substances in leaves, reducing the degree of cell membrane lipid peroxidation, and improving the activity of antioxidant enzymes and photosynthetic capacity, and when the concentration of HIDS is 700mg·L−1, each index is closest to CK treatment.

Key words: Zucchini, Suboptimal temperature, HIDS, Antioxidant, Photosynthetic