磷脂酶D参与冬小麦对干旱胁迫的响应

doi:10.3969/j.issn.1000-6362.2019.04.003

摘要/Abstract

摘要： 磷脂酶D（phospholipase D，PLD）通过水解细胞膜磷脂产生信使物质磷脂酸（PA），并介导多种激素与逆境的信号转导过程。为探讨PLD在参与干旱信号转导调控细胞膜稳定性方面的作用及其途径，采用在培养液中加入PEG-6000模拟干旱胁迫，用PLD抑制剂正丁醇（n-butanol，BA）抑制PLD产生PA，对比PEG+BA处理与PEG处理下小麦抗旱性、膜稳定性以及抗氧化酶类的变化。结果表明，与PEG处理相比，PEG+BA处理下，冬小麦幼苗叶片生长受抑制，净光合速率下降，光合非气孔限制作用增强，细胞膜离子渗漏率显著升高，膜脂过氧化产物丙二醛（malonaldehyde，MDA）升高，抗氧化酶POD活性下降，表明PLD参与干旱胁迫下过氧化物酶（peroxidase，POD）活性的调控。由此揭示出一条潜在的由PLD介导的干旱信号转导途径，即干旱胁迫—PLD激活—POD活性增强—保护膜脂过氧化损伤—提高细胞膜稳定性—植物抗旱性增强。

关键词: 磷脂酶D, 干旱, 信号转导, 过氧化, 抗氧化系统, PLD

Abstract: Phospholipase D (PLD) mediate signaling processes of various hormones and adversities by hydrolyzing phospholipids in cellular membrane and producing phosphotidic acid (PA), a messenger. In order to investigate the roles and pathways of PLD in regulation of the stability of cellular membrane, PEG-6000 was adopted to mimic drought stress, and n-butabol (BA) was applied to inhibit PLD-derived PA. Drought resistance, stability of cellular membrane, antioxidant enzymes of wheat seedlings treated by PEG and PEG added BA were compared. The results showed that inhibition of PLD by BA induced slower seedling growth, lower net photosynthesis rate, higher non-stomatal limitation, higher membrane ion leakage and malonaldehyde (MDA), a peroxidation product, and lower POD activity. The results indicated that PLD played its role in regulating POD activity. Thus, a potential signal transduction pathway under drought stress mediated by PLD was explored: drought stress—activation of PLD—enhancement of POD—impairation of membrane lipids peroxidation damage—improvement of stability of cellular membrane—increasing drought resistance of plants.

Key words: Phospholipase D, Drought, Signal transduction, Peroxidation, Antioxidant enzyme, PLD

王雅静，张欣莹，黄桂荣，封富，钟秀丽. 磷脂酶D参与冬小麦对干旱胁迫的响应[J]. 中国农业气象, 2019, 40(04): 222-229.

WANG Ya-jing, ZHANG Xin-ying, HUANG Gui-rong, FENG Fu, ZHONG Xiu-li. Phospholipase D of Winter Wheat is Involved in Responses to Drought Stress[J]. Chinese Journal of Agrometeorology, 2019, 40(04): 222-229.

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