中国农业气象

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La(Ⅲ)对UV-B辐射胁迫下大豆叶绿素合成与降解影响的机理

彭倩;周青;   

  1. 江南大学生物工程学院环境科学研究室,江南大学生物工程学院环境科学研究室 无锡214036,无锡214036,江南大学工业生物技术教育部重点实验室
  • 出版日期:2007-06-10 发布日期:2007-06-10
  • 基金资助:
    国家自然科学基金(30570323);; 江苏省科技厅高新技术基金(GB2005040)

Effects of La on Leaf Chlorophyll Biosynthesis and Degradation of Soybean(Glycine max) under Ultraviolet-B Stress

PENG Qian1,ZHOU Qing1,2 (1.Laboratory of Environmental Science,School of Biotechnology, Southern Yangtze University,Wuxi 214036,China;2.Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze University)   

  • Online:2007-06-10 Published:2007-06-10

摘要: 采用水培实验方法研究了稀土元素La(Ⅲ)对紫外辐射(UV-B:0.15Wm-2,0.45Wm-2)胁迫下大豆(Glycine max)幼苗的δ-氨基乙酰丙酸(ALA)、胆色素原(PBG)、原叶绿素(酸)(Pchl)、原卟啉IX(Proto-IX)、镁原卟啉(Mg-Proto)等5种叶绿素(Chl)合成中间产物含量及Chl降解关键酶-叶绿素酶(Chlase)活性的影响,从叶绿素的生物合成及降解角度诠释La(Ⅲ)对UV-B辐射胁迫下大豆幼苗叶绿素含量影响的机理。实验结果表明:La(Ⅲ)具有提高叶绿素生物合成中间产物——ALA(δ-氨基乙酰丙酸)、PBG(胆色素原)、Proto-IX(原卟啉IX)、Mg-Proto(镁原卟啉)含量的作用,促进Pchl(原叶绿素)转化为叶绿素,进而缓解UV-B辐射胁迫下中间产物PBG、Proto-IX和Mg-Proto的形成,使叶绿素的生物合成受阻,受阻位点为ALA→PBG。

关键词: La(Ⅲ), UV-B辐射, 叶绿素(Chl)合成与降解

Abstract: Effect of La on the chlorophyll content of Soybean(Glycine max) seedling under elevated ultraviolet-B radiation(UV-B,280-320 nm) was studied.When seedling was pretreated by La(Ⅲ),the content of the interim outcomes of the chlorophyll such as δ-amino-laevulic acid(ALA),proto bile pigment(PBG),proto-porphyrin-IX(Proto-IX) and magnesium protoporphyrin(Mg-Proto) increased,and the process from protochlorophyll to chlorophyll was accelerated.The La(Ⅲ) promoted the protective effect of plant against UV-B stress through delaying the form of the PBG,Proto-IX,Mg-Proto and ALA.Therefore,the chlorophyll biosynthesis was suffocated under the condition of UV-B stress.The restrained location was from ALA to PBG.

Key words: Lanthanum(La), Lanthanum(La), Ultraviolet-B radiation, Chlorophyll biosynthesis and degradation