中国农业气象

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La(Ⅲ)对酸雨胁迫下水稻萌发种子能量代谢的影响

杨维;周青;   

  1. 江南大学工业生物技术教育部重点实验室/江南大学环境与土木工程学院;
  • 出版日期:2010-02-10 发布日期:2010-02-10
  • 基金资助:
    江苏省教育厅高等学校教育教学改革项目(3-26-77);; 江南大学质量工程建设项目(20081218)

Effects of La(Ⅲ) on Energy Metabolism of Rice Seeds under Acid Rain Stress

YANG Wei,ZHOU Qing(Key Laboratory of Industrial Biotechnology,Ministry of Education/School of Environment and Civil Engineering,Jiangnan University,Wuxi 214122,China)   

  • Online:2010-02-10 Published:2010-02-10

摘要: 为探索稀土元素镧La(Ⅲ)对酸雨(AR)胁迫下水稻萌发种子能量代谢的影响,试验采用蒸馏水和La(Ⅲ)浸泡水稻种子,用pH2.5的模拟酸雨处理种子,观测试验条件下水稻萌发种子的呼吸速率、过氧化氢酶(CAT)活性、线粒体蛋白、ATP含量及能荷(EC)。结果表明,随着酸雨胁迫时间的延长,La+AR组和AR组各项指标变化趋势基本一致,水稻种子呼吸速率先下降后上升,在AR胁迫第4天,呼吸速度达到最大,随后又下降。CAT活性和线粒体蛋白含量均呈逐渐上升趋势最后趋于平稳,ATP含量及EC水平先急剧上升,第5天达到最高值后有所下降。La+AR组各项指标均高于AR组,表明La(Ⅲ)浸种可缓解AR胁迫下上述各指标降幅,增强水稻种子对酸雨的抵抗能力,缓解酸雨对种子萌发的伤害。

关键词: 酸雨胁迫, 能量代谢, La(Ⅲ), 水稻种子

Abstract: In order to explore the effects of La(Ⅲ) on the energy metabolism of rice seeds under acid rain stress,the experiment of simulating acid rain(pH2.5) to rice seeds in distilled water and La(Ⅲ) was conducted.The effect of simulated acid rain on respiration rate,activity of CAT,mitochondrial protein,ATP content and Energy Charge(EC) of rice seeds were studied.The results showed that all the treated groups had the same alter trend with acid rain treatment period extending.The respiration rate of rice seeds declined at first then increased,and reached the peak on the forth day,then declined again.The activity of CAT and mitochondrial protein content increased slowly at the beginning.ATP content and EC of rice seeds increased sharply to the peak on the fifth day and then declined.All the targets of the AR+La groups were higher than that of the AR groups.These results indicated that La(Ⅲ) could lighten the damage of acid rain on mitochondria,and enhance resistance of rice seed to acid rain.

Key words: Acid rain stress, Acid rain stress, Energy metabolism, La(Ⅲ), Rice seeds