中国农业气象 ›› 2023, Vol. 44 ›› Issue (04): 285-294.doi: 10.3969/j.issn.1000-6362.2023.04.003

• 农业生态环境栏目 • 上一篇    下一篇

不同地点和复垦方式煤矸石山土壤中丛枝菌根真菌群落变化

李侠,叶诚诚,张永芳,张巽,霍丽娟,苏世鸣   

  1. 1.山西大同大学农学与生命科学学院,大同 037009;2.温州市优质农产品开发服务中心,温州 325000;3.太原科技大学环境科学与工程学院,太原 030024;4.中国农业科学院农业环境与可持续发展研究所/农业农村部农业环境重点实验室,北京 100081
  • 收稿日期:2022-05-17 出版日期:2023-04-20 发布日期:2023-04-15
  • 通讯作者: 叶诚诚,农艺师,主要从事农产品加工与质量安全研究。 E-mail:chchye@cau.edu.cn
  • 作者简介:李侠,E-mail:lixia810504@163.com
  • 基金资助:
    国家自然科学基金(41807130);山西省应用基础研究计划项目(201901D111306);大同市平城区重点研发计划项目(202003);大同市重点研发项目(2020044)

Community Response of Arbuscular Mycorrhizal Fungi to Restoration Managements and Sites in Coal Gangue Hills

LI Xia, YE Cheng-cheng, ZHANG Yong-fang, ZHANG Xun, HUO Li-juan, SU Shi-ming   

  1. 1.College of Agronomy and Life Science, Shanxi Datong University, Datong 037009, China; 2.Wenzhou Good Agri-Products Development Service Center, Wenzhou 325000; 3.School of Environmental Science and Engineering, Taiyuan University of Science and Technology, Taiyuan 030024; 4.Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Environment, Ministry of Agriculture and Rural, Beijing 100081
  • Received:2022-05-17 Online:2023-04-20 Published:2023-04-15

摘要: 以山西省大同市晋华宫人工复垦和自然恢复煤矸石山以及忻州窑自然恢复煤矸石山为研究对象,采用Illumina MiSeq高通量测序技术对三座煤矸石山土壤AM真菌群落组成进行分析,并测定其土壤理化性质,以探究不同地点煤矸石山不同复垦方式下土壤中丛枝菌根真菌(Arbuscular mycorrhizal fungi, AM真菌)群落组成的差异及其土壤影响因子。结果表明:晋华宫人工复垦煤矸石山和忻州窑自然恢复煤矸石山AM真菌多样性指数(Chao指数和香农−威纳指数)存在显著差异,但晋华宫人工复垦和自然恢复煤矸石山AM真菌群落的多样性指数差异不显著。试验共检测到67个AM真菌OTUs,可鉴定到3个AM真菌属级分类单元,其中球囊霉属(Glomus)在晋华宫人工复垦煤矸石山、晋华宫自然恢复煤矸石山和忻州窑自然恢复煤矸石山中均为最优势菌,多样孢囊霉属(Diversispora)、类球囊霉属(Paraglomus)仅出现在晋华宫自然恢复煤矸石山。多元置换方差分析和多维尺度分析结果表明,晋华宫两种恢复方式的煤矸石山之间AM真菌群落组成差异不显著,而晋华宫与忻州窑两个地点的煤矸石山AM真菌群落组成差异显著。AM真菌群落差异与土壤全氮、有机碳含量和AM真菌孢子密度相关。综上,晋华宫人工复垦(10~20a)煤矸石山土壤AM真菌群落可达到与自然恢复(50~60a)煤矸石山较为一致的效果。两个地点煤矸石山土壤AM真菌群落差异较大,土壤全氮、有机碳含量和AM真菌孢子密度是驱动AM真菌群落组成差异的主要因子。

关键词: 煤矸石山, 丛枝菌根真菌群落, 球囊霉属, Illumina MiSeq高通量测序

Abstract: Illumina MiSeq high-throughput sequencing approach was applied to investigate arbuscular mycorrhizal fungi (AM fungi) community and relevant soil factors were analyzed in coal gangue hills with different restoration patterns (i.e., artificial restoration and natural restoration coal gangue hill of Jinhua palace in Datong City; natural restoration coal gangue hill of Xinzhou kiln). The results showed: α-diversity (Chao index and Shannon-Weiner index) of AM fungi community differed significantly between artificial restoration coal gangue hill of Jinhua palace and natural restoration coal gangue hill of Xinzhou kiln, but comparable α-diversity was observed between artificial restoration and natural restoration coal gangue hill of Jinhua palace. A total of 67 AM fungal OTUs assigned to 3 genus were recovered with Glomus being the predominant genus in all three coal gangue hills, while Diversispora and Paraglomus populations were exclusive to natural restoration coal gangue hill of Jinhua palace in Datong city. Integrated the permutational multivariate analysis and nonmetric multidimensional scaling analysis suggested that AM fungi community were similar between the two restorations of Jinhua palace coal gangue hill, while the community significantly differed between coal gangue hill of Jinhua palace and Xinzhou kiln. The AM fungal community appeared to significantly correlate with soil total nitrogen, soil organic matter and AM fungi spore density. In conclusion, artificial restoration for 10-20 years can exert a comparable effect on AM fungal community as natural restoration 50-60 years in coal gangue hill of Jinhua palace. AM fungal community of the two locations showed great variance, and soil total nitrogen, soil organic matter and soil AM fungi spore density were the main driving factors.

Key words: Coal gangue hill, Arbuscular mycorrhizal fungi community, Glomus, Illumina MiSeq high-throughput sequencing