中国农业气象

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Ce(Ⅲ)对UV-B辐射胁迫下大豆幼苗N、P含量的影响

任晓晓;周青;   

  1. 江南大学工业生物技术教育部重点实验室/江南大学环境与土木工程学院;
  • 出版日期:2008-08-10 发布日期:2008-08-10
  • 基金资助:
    国家发改委稀土专项基金(IFZ20051210)

Effects of Cerium(Ⅲ) on Nitrogen and Phosphor Absorption in Soybean Seedling under Supplemental UV-B Radiation Stress

REN Xiao-xiao,ZHOU Qing(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University/Environment and Civil Engineering,Jiangnan University,Wuxi 214122,China)   

  • Online:2008-08-10 Published:2008-08-10

摘要: 在温室模拟UV-B胁迫下,采用水培法研究稀土Ce(Ⅲ)对大豆(Glycine max)幼苗N、P吸收以及N代谢相关酶活性的影响。结果显示:UV-B辐射胁迫下,大豆幼苗叶片硝酸还原酶(Nitrate reductase,NR)活性、硝态氮含量、磷含量较对照组均有显著下降,说明UV-B辐射严重抑制NR活性、使叶片内硝态氮(NO3-)和磷含量减少,而且高强度、长时间辐射时的抑制作用更大;而适宜浓度CeCl3(20mg.L-1)处理能提高叶片中NO3-和磷含量,缓解T1、T2胁迫下NO3-和磷含量的减少。

关键词: Ce(Ⅲ), UV-B辐射, 大豆幼苗, NR, 硝态氮,

Abstract: The effect of Cerium(Ⅲ) on metabolism enzyme activity of nitrogen and absorption of nitrogen and phosphor in soybean seedlings exposed to two levels of simulated ultraviolet-B radiation stress(UV-B,280-320 nm) was studied in hydroponics under greenhouse conditions.The results showed that the nitrate reductase activity,nitrate nitrogen and phosphor content in soybean seedlings' leaves in UV-B treatment significantly decreased compared to the check.Therefore,it was indicated that nitrate reductase activity was seriously inhibited by UV-B stress which led to a decrease of the nitrate nitrogen and phosphor content.The inhibitory action was stronger,as the UV-B stress was with high intensity and lasted longer time.The suitable concentration by 20mg·L-1 of CeCl3 could promote absorption nitrate nitrogen and phosphor content in soybean seedlings to alleviate the decrease of nitrate nitrogen and phosphor content.

Key words: Cerium(Ⅲ), Cerium(Ⅲ), UV-B radiation, Soybean seedlings, Nitrate reductase(NR), Nitrate nitrogen, Phosphor