中国农业气象 ›› 2013, Vol. 34 ›› Issue (05): 563-568.doi: 10.3969/j.issn.1000-6362.2013.05.010

• 论文 • 上一篇    下一篇

基于叶绿素荧光参数的栓皮栎叶片PSⅡ失活高温指标

陈景玲,宋晓明,王谦,王昆,赵勇   

  1. 河南农业大学,郑州450002
  • 收稿日期:2013-03-20 出版日期:2013-10-20 发布日期:2014-01-03
  • 作者简介:陈景玲(1964-),女,河南洛阳人,副教授,主要从事植物与环境传热研究。Email:chenjingling5@163.com
  • 基金资助:
    国家自然科学基金项目(31071321)

High Temperature Index of PSⅡ Inactivation According to Chlorophyll Fluorescence of Quercus variabilis Leaves

CHEN Jing ling,SONG Xiao ming,WANG Qian,WANG Kun,ZHAO Yong   

  1. Henan Agricultural University,Zhengzhou450002,China
  • Received:2013-03-20 Online:2013-10-20 Published:2014-01-03

摘要: 利用Mini PAM叶绿素荧光仪测定离体栓皮栎叶片高温胁迫下光系统Ⅱ(PSⅡ)最大量子产量Fv/Fm和实际量子产量ΔF/Fm’,以研究栓皮栎叶片高温胁迫下PSⅡ失活的温度指标。试验设35~55℃共10个高温处理,处理时间5~85min。结果表明,(1)PSⅡ最大量子产量Fv/Fm和实际量子产量ΔF/Fm’均随胁迫温度升高和处理时间延长而呈下降趋势,ΔF/Fm’的下降过程呈Logistic曲线形式;(2)高温胁迫显著影响栓皮栎叶片的光合作用,ΔF/Fm’降低50%的温度T50是PSⅡ失活的临界温度指标,可作为确定高温胁迫温度指标的特征点,栓皮栎的高温胁迫叶温指标为42℃。研究结果可用于栓皮栎高温胁迫强度和持续时间的定量分析,并为确定其它作物农业气象指标提供方法。

关键词: 高温胁迫, 栓皮栎, PSⅡ, 叶绿素荧光, 量子产量

Abstract: Using chlorophyll fluorescence instrument Mini PAM, authors measured the Quercus variabilis leaves maximal and actual chlorophyll fluorescence quantum yield of photo system Ⅱ(Fv/Fm and ΔF/Fm’) in order to study the temperature of photo system Ⅱ inactivation of Quercus variabilis leaves on high temperature stress.There were 10 high temperature treatments of 35~55℃ with treating time 5~85min.The results showed that (1) both Fv/Fm and ΔF/Fm’ decreased with increase of heat stress temperature and increase of high temperature treating time; and the process of that ΔF/Fm’decreased could be simulated with Logistic curves; (2) High temperature stress clearly influenced photosynthesis of Quercus variabilis leaves; the temperature of that ΔF/Fm’ decreased 50%(T50) was limit temperature index of photo system Ⅱ inactivation, and could be taken as characteristic point of high temperature stress; the high temperature tress index of Quercus variabilis was determined as 42℃. This paper is useful for analyzing the intensity and lasting time of high temperature stress of Quercus variabilis leaves, and providing methods of determining agricultural meteorology index of other plants.

Key words: High temperature stress, Quercus variabilis, PSⅡ, Chlorophyll fluorescence, Quantum yield