Effect of Continuous Light with Blue and Red LED on Growth and Quality of Different Lettuce Cultivars

doi:10.3969/j.issn.1000-6362.2018.07.003

Abstract

Abstract: In order to explore lettuce responses to whole growth period continuous light and diverse responses among cultivars, an experiment was conducted in an environmentally-controlled plant factory equipped with red plus blue LED lamps to investigate the effects of normal lighting (12h/12h, 6：00?18：00, NL) and continuous lighting (24h/0h, 6：00?6：00, CL) on the yield and quality of five hydroponic lettuce cultivars. The results showed that the shoot fresh weights of five lettuce cultivars except ‘Dawei’ cultivar were significantly improved under CL treatments compared with NL treatment. ‘Lvluo’ cultivar showed the greatest amplification among five cultivars. At early growth stage, the leaf chlorophyll contents of five cultivars grown under CL were significantly higher than those grown under NL. While no significant difference of five cultivars’ leaf chlorophyll contents was observed between NL and CL treatments at harvest. Compared to NL treatment, CL treatment significantly increased the soluble sugar, total phenolic and flavonoid contents of five cultivars and anthocyanin contents of two purple cultivars.CL treatment slightly or significantly increased total ascorbic acid content of all lettuce cultivars except ‘Lvluo’ cultivar, and mainly increased in reduction state. CL had no significant effect on dehydroascorbic acid of five cultivars. In short, CL could significantly enhance the yield and biosynthesis of soluble sugar and antioxidant substance. The adaptability of five lettuce cultivars to CL was different, and ‘Lvluo’ cultivar was the most adaptable cultivar. CL during whole growth period has the potential to enhance yield and quality of lettuce in plant factory production.

Key words: Photoperiod, Leaf color, Biomass, Antioxidant substances, Ascorbic acid, LED

ZHA Ling-yan, LIU Wen-ke. Effect of Continuous Light with Blue and Red LED on Growth and Quality of Different Lettuce Cultivars[J]. Chinese Journal of Agrometeorology, 2018, 39(07): 453-461.

References

[1] Sysoeva M I,Markovskaya E F,Shibaeva T.Plants under continuous light:a review[J].Plant Stress,2010,4:5-17. [2] Velez-Ramirez A I,Van I W,VreugdenhilD,et al.Plants under continuous light[J].Trends in Plant Science,2011,16(6):310-318. [3] Ohyama K,OmuraY,Kozai T.Effects of air temperature regimes on physiological disorders and floral development of tomato seedlings grown under continuous light[J].Hortscience, 2005,40(5):1304-1306. [4] Demers D A,Dorais M,Wien C H,et al.Effects of supplemental light duration on greenhouse tomato (Lycopersicon esculentum Mill.) plants and fruit yields[J].Scientia Horticulturae,1998, 74(4):295-306. [5] 李海云,韩国徽,任秋萍,等.不同光周期对黄瓜幼苗生长的影响[J].西北农业学报,2009,18(3):201-203 Li H Y,Han G H,Ren Q P,et al.The effect of different photoperiod on growth of cucumber seedling[J].Acta Agriculturae Boreali- occidentalis Sinica,2009,18(3):201-203. (in Chinese) [6] Zhou W L,Liu W K,Yang Q C.Reducing nitrate content in lettuce by pre-harvest continuous light delivered by red and blue light-emitting diodes[J].Journal of Plant Nutrition,2013, 36(3):481-490. [7] Urbonavi?iu?te? A,Samuoliene? G,Brazaityte? A,et al.The possibility to control the metabolism of green vegetables and sprouts using light emitting diode illumination[J]. Sodininkyste? Ir Darz?ininkyste?,2008,27(2):83-92. [8] Samuolien? G,Brazaityt? A,Sirtautas R,et al.LED illumination affects bioactive compounds in romaine baby leaf lettuce[J]. Journal of the Science of Food & Agriculture,2013,93(13): 3286. [9]张欢,章丽丽,李薇,等.不同光周期红光对油葵芽苗菜生长和品质的影响[J].园艺学报,2012,39(2):297-304. Zhang H,Zhang L L,Li W,et al.Effects of photoperiod under red led on growth and quality of sunflower sprouts[J].Acta Horticulturae Sinica,2012,39 (2):297-304.(in Chinese) [10]Wu M C,Hou C Y,Jiang C M,et al.A novel approach of LED light radiation improves the antioxidant activity of pea seedlings[J].Food Chemistry,2007,101(4):1753-1758. [11]周晚来,刘文科,闻婧,等.短期连续光照下水培生菜品质指标变化及其关联性分析[J].中国生态农业学报,2011,19(6): 1319-1323. Zhou W L,Liu W K,Wen J,et al.Changes in and correlation analysis of quality indices of hydroponic lettuce under short- term continuous light[J].Chinese Journal of Eco-Agriculture, 2011,19(6):1319-1323.(in Chinese) [12]Pettersen R I,Torre S,Gisler?d H R.Effects of leaf aging and light duration on photosynthetic characteristics in a cucumber canopy[J].Scientia Horticulturae,2010,125(2):82-87. [13]Van Gestel N,Nesbit A E,Green C,et al.Continuous light may induce photosynthetic downregulation in onion-consequences for growth and biomass partitioning[J].Physiologia Plantarum, 2010,125(2):235-246. [14]Gillespie K M,Ainsworth E A.Measurement of reduced, oxidized and total ascorbate content in plants[J].Nature Protocols,2007, 2(4):871-874. [15]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007. Cao J K,Jiang W B,Zhao Y M.Experiment guidance of postharvest physiological and biochemistry of fruits and vegetables[M].Beijing:China Light Industry Press,2007.(in Chinese) [16]Koontz H V,Prince R P.Effect of 16 and 24 hours daily radiation(light) on lettuce growth[J].Hortscience A Publication of the American Society for Horticultural Science,1986, 21(1):123. [17]余意,刘文科.弱光条件下光质和光周期对水培生菜生长与品质的影响[J].中国农业气象,2015,36(6):739-745 Yu Y,Liu W K.Influence of light quality and photoperiod on growth and nutritional quality of three leaf-color lettuce cultivars under weak light[J].Chinese Journal of Agrometeorology, 2015,36(6):739-745.(in Chinese) [18]Velez-Ramirez A I,Heuvelink E,Van Ieperen W,et al.Contin- uous light as a way to increase greenhouse tomato production:expected challenges[J].Acta Horticulturae,2012, 956(1):51-57. [19]KitayaY,Niu G,Kozai T,et al.Photosynthetic photon flux, photoperiod, and CO2 concentration affect growth and morp- hology of lettuce plug transplants[J].Hortscience A Publication of the American Society for Horticultural Science,1998,33(6): 58-62. [20]吴泽英,薛占军,高志奎.全光期LED白光对番茄植株干物质累积及光合性能的影响[J].河北农业大学学报,2015,38 (4):56-61 Wu Z Y,Xue Z J,Gao Z K.Effects of continuous photoperiod of white LED illumination on dry matter accumulation and photosynthetic performance of tomato seedling[J].Journal of Agricultural University of Hebei,2015,38(4):56-61.(in Chinese) [21]Langton F A,Adams S R,Cockshull K E.Effects of photoperiod on leaf greenness of four bedding plant species [J].Journal of Horticultural Science,2003,78(3):400-404. [22]童哲,赵玉锦,王台,等.植物的光受体和光控发育研究[J].植物生态学报(英文版),2000,42(2):111-115. Tong Z,Zhao Y J,Wang T,et al.Photoreceptors and light- regulated development in plants[J].Acta Botanica Sinica, 2000,42(2):111-115.(in Chinese) [23]李世栋,刘建辉,张秉奎,等.不同昼温对厚皮甜瓜幼苗生长与生理特性的影响[J].西北农林科技大学学报:自然科学版,2007,35(4):163-167. Li S D,Liu J H,Zhang B K,et al.Effects of different day- temperature on the growth and physiology characteristics of muskmelon seedling[J].Journal of Northwest A and F University (Natural Science Edition),2007,35(4):163-167.(in Chinese) [24]陈敏,李海云.不同光周期对茄子幼苗生长的影响[J].北方园艺,2010(16):53-55 Chen M,Li H Y.Effect of different photoperiod on the growth of eggplant seedling[J].Northern Horticulture,2010,(16):53-55. (in Chinese) [25]彭姣凤,张磊.光氧化的成因及其削减机制[J].生命科学研究,2000,4(2):84:84-91. Peng J F,Zhang L.Causes for the formation and reduction mechanisms of photooxidation[J].Life Science Research,2000, 4(2):84-91.(in Chinese) [26]严妍.温度和光周期对水培生菜生长的影响及生长模型初探[D].武汉:华中农业大学,2008. Yan Y,Effects of temperature and photoperiod on the growth of hydroponic lettuce and primary research on growth modelling[D].Wuhan:Huazhong Agricultural University,2008. (in Chinese) [27]Havsteen B H.The biochemistry and medical significance of the flavonoids[J].Pharmacology & Therapeutics,2002,96: 67- 202. [28]李跃,刘延吉.果实花青苷代谢机制及调控技术研究[J].安徽农业科学,2007,35(16):4755-4756. Li Y,Liu Y J.Research on metabolism mechanism of fruit anthocyanin and control technique[J].Journal of Anhui Agricultural Sciences,2007,35(16):4755-4756.(in Chinese) [29]马春花,李明军,李翠英,等.不同抗性苹果砧木叶片抗坏血酸代谢对干旱胁迫的响应[J].西北植物学报,2011,31(8): 1596-1602 Ma C H,Li M J,Li C Y,et al.Response of ascorbic acid metabolism in apple rootstocks leaves under drought stress[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31 (8):1596-1602.(in Chinese) [30]邹礼平,陈锦华.植物抗坏血酸的合成和代谢以及相关酶基因的调控[J].植物生理学报,2009,45(9):925-930. Zou L P,Chen J H.Biosynthesis and metabolism of ascorbic acid and regulation of related genes in plants[J].Plant Physiology Communications,2009,45(9):925-930.(in Chinese) [31]俞乐,刘拥海,李充壁.植物抗坏血酸合成的关键酶:L-半乳糖内酯脱氢酶(GLDH)[J].植物生理学报,2009,45(2):183- 186. Yu L,Liu Y H,Li C B.The key enzyme for ascorbic acid biosynthesis in plants:L-galactono-1,4-lactone dehydrogenase (GLDH)[J].Plant Physiology Communications,2009,45(2):183- 186. (in Chinese) [32]Tamaoki M,Mukai F,Asai N,et al.Light-controlled expression of a gene encoding L-galactono-γ-lactone dehydrogenase which affects ascorbate pool size in Arabidopsis thaliana, [J].Plant Science,2003,164(6):1111-1117. [33]李亚华,陈龙,高荣广,等.LED光质对茄子果实品质及抗氧化能力的影响[J].应用生态学报,2015,26(9):2728-2734. Li Y H,Chen L,Gao R G,et al.Effects of LED qualities on quality and antioxidation capacity of eggplant fruits[J]. Chinese Journal of Applied Ecology,2015,26 (9):2728-2734. (in Chinese) [34]Demers D A,Dubé S,Yelle S, et al.Effects of continuous lighting and light spectral composition on photosynthesis and related processes of greenhouse grown tomato and pepper plants[J].Hortscience A Publication of the American Society for Horticultural Science,1995,30(4):778. [35]Velez-Ramirez A I,Van I W,Vreugdenhil D,et al.A single locus confers tolerance to continuous light and allows substantial yield increase in tomato[J].Nature Communications, 2014,5:45-49.