Temperature Index of Quercus variabilis Leaf PSII Activity Damage under High Temperature Combined with Drought

doi:10.3969/j.issn.1000-6362.2019.05.005

Abstract

Abstract: The temperature index of Quercus variabilis seedling in high temperature and drought stress was determined using method of soil water controlling in pot and temperature controlling in climate box. Soil volumetric humidity >14.5% was set as no drought stress (T0), 12.5%?14.5% was set as mild drought (T1), 9.5%?11.5% was set as moderate drought (T2) and 5.5%?7.5% was set as severe drought (T3). The temperature was controlled at 35，37，39，…，49℃ and at any temperature of which keeping treating time as 1，2，…，6h. The parameters of Chlorophyll Fluorescence measured were Fv/Fm, Y(II), ETR, Y(NPQ) and Y(NO), which were used to determine stress temperature index. The results showed: (1) from mild, moderate to severe drought, the changing curves of Fv/Fm, Y(II) adapt to temperature decreased more rapidly, which indicated the drought serious level had obvious significance on temperature stress index. (2)The temperature indices of different drought levels indicating PSII damaging determined by Fv/Fm were 51.6?57.5℃; The temperature indices of different drought levels indicating PSII efficiency of photosynthesis determined by Y(II) were 40.7?52.5℃; (3) Regulatory heat dissipation Y(NPQ) began to rise at 45℃ by T0 and T1, and at 43℃ by T2, 41℃ by T3; (4) Nonregulatory heat dissipation Y(NO) of T0 hardly rise, and T1 began to rise at 47℃, T2 and T3 began to rise at 45℃. The results above indicated the temperature indices of PSII damaged and photosynthetic efficiency decreased under superimposed drought conditions were significantly different from those without drought. The more drought, the lower the stress temperature index. Both active and passive heat dissipations of Quercus variabilis were increased by drought stress, and the temperature at which excess light energy of active dissipation began to increase was lower than that at which passive heat dissipation began to increase.

Key words: Quercus variabilis, Drought stress, Chlorophyll fluorescence, Photo system damage, Temperature index

WANG Qian, CHEN Ling-shu, ZHAO Wei, CHEN Jing-ling, ZHANG Jin-shong, YANG Xi-tian,ZHAO Yong. Temperature Index of Quercus variabilis Leaf PSII Activity Damage under High Temperature Combined with Drought[J]. Chinese Journal of Agrometeorology, 2019, 40(05): 308-316.

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