Chinese Journal of Agrometeorology ›› 2022, Vol. 43 ›› Issue (07): 538-550.doi: 10.3969/j.issn.1000-6362.2022.07.003

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Effects of Fertilization Regimes on Soil Alkaline Phosphatase Gene Encoding Microbial Community under the Integrated Planting and Breeding System in the Vegetable Field

HE Yu, LV Wei-guang, ZHENG Xian-qing, ZHANG Han-lin, LI Shuang-xi, ZHANG Juan-qin, ZHANG Hai-yun, BAI Na-ling   

  1. 1. Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403; 2. Faculty of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China; 3. Shanghai Agri-environmental and Cultivated Land Conservation of Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Shanghai 201403; 4. Shanghai Agri-environmental Protection Monitoring station, Shanghai 201403; 5. Shanghai Key Laboratory of Protected Horticulture Technology, Shanghai 201403
  • Received:2021-09-18 Online:2022-07-20 Published:2022-07-20

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Abstract: A field experiment was systematically conducted, using Illumina MiSeq high-throughput sequencing, to analyze the impact of four different fertilizer application regimes (CK, no fertilization; CF, conventional chemical fertilizer; OF, organic fertilizer; MF, organic-inorganic mixed fertilizer) on the soil physicochemical properties, alkaline phosphatase (ALP), microbial biomass phosphorus (MBP), and phoD (gene encoding alkaline phosphatase) microbial community in 0-20cm soil layer at cauliflower harvest season, thus identifying the underlying mechanism of effects of different fertilization regimes on the structure and diversity of phoD microbe under integrated planting and breeding system in vegetable field. The results showed that: (1) compared to CK, OF significantly increased the contents of soil organic matter, total nitrogen, available phosphorus and Ca by 47.83%, 38.46%, 104.81% and 69.21%, respectively (P<0.05); both OF and MF significantly increased ALP activity. The MBP content in CF and OF was improved by 56.12% and 195.16%, respectively, compared with CK; the MBP content in OF was the highest (105.40mg·kg−1). (2) Pseudomonas was the dominant genus under different fertilization treatments. CF and MF significantly reduced the relative abundance of Pseudomonas by 33.39% and 45.52%, respectively, compared with CK. Fertilization decreased the Chao1 index, and MF increased both the diversity (Simpson index) and evenness (Simpsoneven index) of soil phoD microorganisms. (3) MBP, AP, and ALP were the key environmental factors affecting the phoD microbial community structure. There was no significant correlation between phoD microbial α diversity indexes and soil properties. Therefore, under the integrated planting and breeding system in vegetable field, different fertilization treatments changed soil physicochemical and biological properties, and thus drove the changes of composition, structure and diversity of soil phoD microbial community.

Key words: Integrated planting and breeding system in the vegetable field, Fertilization regimes, Alkaline phosphatase, phoD gene, High-throughput sequencing