孕穗开花期持续低温对不同熟期水稻气孔导度的影响

doi:10.3969/j.issn.1000-6362.2016.06.008

摘要/Abstract

摘要： 分别以 9023、9035、9036为早熟、中熟、晚熟水稻的代表品种，应用人工气候箱在孕穗开花期进行低温处理，旨在分析沈阳地区孕穗开花期不同强度低温对不同熟期水稻叶片气孔导度的影响。低温设置以前一天相同时刻的温度值为基准，设比外界温度低3℃（D3）和低5℃（D5）两个处理水平，持续处理5d，以自然条件下生长的水稻为对照（CK），利用LI-6400光合测定仪测定水稻倒2叶的CO2响应曲线和光响应曲线。结果表明：（1）CO2响应过程中，与CK相比，D3和D5的持续低温处理皆可导致9035和9036的气孔导度显著降低，而9023（早熟）仅D5的持续低温导致其气孔导度降低；（2）光响应过程中，与CK相比，D3和D5的持续低温处理使9035（中熟）和9036（晚熟）气孔导度降低，且降低幅度随低温强度的增强而增大，但持续低温处理显著增加了9023（早熟）的气孔导度（P<0.01）；（3）气孔限制是引起9035（中熟）遭遇低温后光合速率下降的主要原因，而9023（早熟）和9036（晚熟）光合速率的变化主要由非气孔限制导致。说明中熟（9035）和晚熟（9036）水稻品种对低温处理响应较敏感，而早熟（9023）品种对低温不敏感。

关键词: 孕穗开花期, 持续低温, 气孔导度, 气孔限制

Abstract: The experiment was conducted in climate chamber to explore the effects of different intensities of low temperature on stomatal conductance of rice with different maturity at booting and blooming stages in Shenyang region. 9023, 9035, 9036 were chosen as representative early, middle and late maturity varieties, respectively. Two temperature treatments were stetted for 5 days, which were 3℃(D3) and 5℃(D5) lower than outside temperature of the last day, CO2 and light response curves of second leaf from top were measured for treatment and control plants (grown under natural conditions as control, mark CK) by using LI-6400 photosynthesis analyzer. The results showed that:(1) during CO2 response, the leaf stomatal conductance of D3 and D5 treatment plants showed a significant decrease compared with that of CK plants for 9035(middle) and 9036(late) maturity varieties, while decrease for 9023(early) maturity variety only in D5 treatment. (2) During light response, the leaf stomatal conductance of treatment plants showed the similar variation with the results of (1) compared with that of CK plants for 9035(middle) and 9036(late) varieties, but increase for 9023(early) maturity variety in D3 and D5 treatment(P<0.01). (3) Stomatal limitation mechanism is the main mechanism that leads to decrease of photosynthetic induced by low temperature for 9035(middle) variety, whereas, the decline in photosynthetic rate is mainly due to non-stomatal limitation for 9023(early) and 9036(late) maturity varieties. 9035(middle) and 9036(late) varieties are more sensitive than 9023(early) maturity variety to low temperature.

Key words: Booting and blooming stage, Consecutive low temperature, Stomatal conductance, Stomatal limitation

马熙达，任传友,王艳华，徐一丹，赵东妮，陈伟，杨斌，田平. 孕穗开花期持续低温对不同熟期水稻气孔导度的影响[J]. 中国农业气象, 2016, 37(06): 682-690.

MA Xi-da, REN Chuan-you, WANG Yan-hua, XU Yi-dan, ZHAO Dong-ni, CHEN Wei, YANG Bin, TIAN Ping. Effects of Consecutive Low Temperature on Stomatal Conductance of Rice with Different Maturity Periods at Booting and Blooming Stages in Shenyang Region[J]. Chinese Journal of Agrometeorology, 2016, 37(06): 682-690.

参考文献

[1]马树庆,王琪,王春乙,等.东北地区水稻冷害气候风险度和经济脆弱度及其分区研究[J].地理研究,2011,30(5):931-938. Ma S Q,Wang Q,Wang C Y,et al.Climate risk and economy vulnerability of rice chilling damage and division in Northeast China[J].Geographcal Research, 2011,30(5):931-938.(in Chinese) [2]胡春丽,李辑,林蓉,等.东北水稻障碍型低温冷害变化特征及其与关键生育期温度的关系[J].中国农业气象,2014, 35(3):323-329. Hu C L,Li J,Lin R,et al.Characters of sterile-type chilling damage in rice and its relationship with temperature at key growth stage in Northeast China[J].Chinese Journal of Agrometeorology, 2014,35(3): 323-329. (in Chinese) [3] 王冬妮,郭春明,刘实,等.吉林省水稻延迟型低温冷害气候风险评价与区划[J].气象与环境学报,2013,29(1):103-107． Wang D N,Guo C M,Liu S,et al.Evaluation and division on climatic risk of rice delayed cool damage in Jilin province[J]. Journal of Meteorology and Environment, 2013, 29(1): 103-107. (in Chinese) [4]朱海霞,王秋京,闫平,等.孕穗抽穗期低温处理对黑龙江省主栽水稻品种结实率的影响[J].中国农业气象,2012, 33(2):304-309. Zhu H X,Wang Q J,Yan P,et al.Effect of low temperature at heading stage on seed setting rate of major rice varieties in Heilongjiang Province [J].Chinese Journal of Agrometeorology, 2012,33(2): 304-309.(inChinese) [5]宋广树,孙忠富,孙蕾,等.东北中部地区水稻不同生育时期低温处理下生理变化及耐冷性比较[J].生态学报,2011, 31(13):3788-3795． Song G S,Sun Z F,Sun L,et al.Comparison between physiological properties and cold tolerance under low temperature treatment during different growing stages of rice in northeast central region of China[J].Acta Ecologica Sinica, 2011, 31(13): 3788- 3795.(in Chinese) [6]Warren C R,Dreyer E.Temperature response of photosynthesis and internal conductance to CO2:results from two independent approaches[J]. Journal of Experimental Botany,2006,57 (12): 3057-3067. [7]高春娟,夏晓剑,师恺,等.植物气孔对全球环境变化的响应及其调控防御机制[J].植物生理学报,2012,48(1):19-28. Gao C J,Xia X J,Shi K,et al.Response of stomata to global climate changes and the underlying regulation mechanism of stress responses[J].Plant Physiology Journal,2012,48 (1): 19-28.(in Chinese) [8]张玉屏,朱德峰,林贤青,等.高温对水稻剑叶生长和气孔导度影响[J].江西农业大学学报,2012,34(1):1-4. Zhang Y P,Zhu D F,Lin X Q,et al.Effect of high temperature stress on leaf growth and stomatal conductance in rice[J].Acta Agriculturae Universitatis Jiangxiensis,2012,34(1): 1-4.(in Chinese) [9]谢晓金,李秉柏,李映雪,等.抽穗期高温胁迫对水稻产量构成要素和品质的影响[J].中国农业气象,2010,31(3):411-415. Xie X J,Li B B,Li Y X,et al.Effects of high temperature stress on yield components and grain quality during heading stage[J].Chinese Journal of Agrometeorology, 2010, 31(3): 411-415.(in Chinese) [10]彭世彰,徐俊增,丁加丽. 控制灌溉水稻气孔导度变化规律试验研究[J].农业工程学报,2005,21(3):1-5. Peng S Z,Xu J Z,Ding J L.Variations of stomatal conductance of rice under contolled irrigation[J]. Transactions of the CSAE,2005,21(3):1-5. (in Chinese) [11]赵黎明,李明,郑殿峰,等.灌溉方式与种植密度对寒地水稻产量及光合物质生产特性的影响[J].农业工程学报, 2015,31(6):159-169. Zhao L M,Li M,Zheng D F,et al.Effects of irrigation methods and rice planting densities on yield and photosynthetic characteristics of matter production in cold area[J]. Transactions of the CSAE,2015, 31(6):159-169.(in Chinese) [12]徐晨,凌风楼,徐克章,等.盐胁迫对不同水稻品种光合特性和生理生化特性的影响[J].中国水稻科学,2013, 27(3):280-286. Xu C,Ling F L,Xu K Z.et al.Effect of salt stress on photosynthetic characteristics and physiological and biochemical traits of different rice varieties[J].Chin J Rice Sci, 2013, 27(3):280- 286. (in Chinese) [13]刘晓龙,徐晨,徐克章,等.盐胁迫对水稻叶片光合作用和叶绿素荧光特性的影响[J].作物杂志,2014,25(2):88-92. Liu X L,Xu C,Xu K Z,et al.Effects on characteristics of photosynthesis and chlorophyll fluorescence of rice under salt stress[J]. Crops, 2014,25(2):88-92.(in Chinese) [14]李健陵,霍治国,吴丽姬,等.孕穗期低温对水稻产量的影响及其生理机制[J].中国水稻科学,2014,28(3):277-288. Li J L,Huo Z G,Wu L J,et al.Effects of low temperature on grain yield of rice and its physiological mechanism at the booting stage[J].Chin J Rice Sci,2014, 28(3):277-288.(in Chinese) [15]李霞,戴传超,程睿,等.不同生育期水稻耐冷性的鉴定及耐冷性差异的生理机制[J].作物学报,2006,32(1):76-83. Li X,Dai C C,Cheng R,et al.Identification for cold tolerance at different growth stages in rice (Oryza sativa L.)and physiological mechanism of differential cold tolerance[J]. Acta Agronomica Sinica,2006,32(1): 76-83.(in Chinese) [16]方修琦,王媛,朱晓禧.气候变暖的适应行为与黑龙江省夏季低温冷害的变化[J].地理研究,2005,24(5):664-672. Fang X Q,Wang Y,Zhu X X.Change of cool summer hazard under an adaptation behavior to the climate warming in Heilongjiang Province,Northeast China[J]. Geographical Research, 2005, 24(5): 664-672.(in Chinese) [17]曲辉辉,姜丽霞,朱海霞,等.孕穗期低温对黑龙江省主栽水稻品种空壳率的影响[J].生态学杂志,2011,30(3):489-493． Qu H H,Jiang L X,Zhu H X,et al.Effects of low temperature at booting stage on the percentage of unfilled grains of major rice varieties in Heilongjiang Province[J].Chinese Journal of Ecology,2011,30 (3): 489-493.(in Chinese) [18]邓化冰,车芳璐,肖应辉,等.开花期低温胁迫对水稻花粉性状及剑叶理化特性的影响[J].应用生态学报,2011, 22(1):66-72. Deng H B,Che F L,Xiao Y H,et al.Effects of low temperature stress during flowering period on pollen characters and flag leaf physiological and biochemical characteristics of rice[J].Chinese Journal of Applied Ecology, 2011,22(1):66-72.(in Chinese) [19]姜丽霞,李帅,闫平,等.黑龙江水稻孕穗期障碍型冷害及其对产量的影响[J].中国农业气象,2009,30(3):463-468. Jiang L X,Li S,Yan P,et al.Sterile type cool injury of rice during booting stage and its impacts on rice yield in Heilongjiang province[J].Chinese Journal of Agrometeorology, 2009, 30(3): 463-468.(in Chinese) [20]王海珍,韩路,徐雅丽,等.不同温度下灰胡杨叶片气孔导度对光强响应的模型分析[J].生态环境学报,2015,24(5):741-748. Wang H Z,Han L,Xu Y L,et al.Model analysis of the stomatal conductance response to light in populus pruinosa at different temperatures in the Taklimakan Desert[J]. Ecology and Environmental Sciences,2015,24(5): 741-748. (in Chinese) [21]李永秀,娄运生,张富存.冬小麦气孔导度模型的比较[J].中国农业气象,2011,32(1):106-110. Li Y X,Lou Y S,Zhang F C.Comparison of stomatal conductance models for winter wheat[J]. Chinese Journal of Agrometeorology, 2011,32(1): 106-110. (in Chinese) [22] 王建林,于贵瑞,王伯伦,等.北方粳稻光合速率、气孔导度对光强和CO2浓度的响应[J].植物生态学报,2005,29(1):16-25. Wang J L,Yu G R,Wang B L,et al.Response of photosynthetic rate and stomatal conductance of rice to light intensity and CO2 concentration in Northern China[J].Acta Phytoecologica Sinica, 2005, 29(1):16-25.(in Chinese) [23]王建林,温学发.气孔导度对CO2浓度变化的模拟及其生理机制[J].生态学报,2010,30(17):4815-4820. Wang J L,Wen X F.Modeling the response of stomatal conductance to variable CO2 concentration and its physiological mechanism[J].Acta Ecologica Sinica, 2010, 30(17): 4815-4820. (in Chinese) [24]许大全.光合作用气孔限制分析中的一些问题[J].植物生理学通讯,1997,33(4):241-244. Xu D Q.Some problems in stomatal limitation analysis of photosynthesis[J].Plant Physiology Communications, 1997,33 (4): 241-244.(in Chinese) [25]丁雷,李英瑞,李勇,等.梯度干旱胁迫对水稻叶片光合和水分状况的影响[J].中国水稻科学,2014,28(1):65-70. Ding L,Li Y R,Li Y,et al.Effects of drought stress on photosynthesis and water status of rice leaves[J].Chin J Rice Sci,2014,28(1):65-70.(in Chinese) [26]王春艳,庞艳梅,李茂松,等.干旱胁迫对大豆气孔特征和光合参数的影响[J].中国农业科技导报,2013,15(1):109-115. Wang C Y,Pang Y M,Li M S,et al.Effects of drought stress on soybean stomatal characteristics and photosynthetic parameter[J].Journal of Agricultural Science and Technology, 2013,15(1):109-115.(in Chinese) [27]杨泽粟,张强,郝小翠.自然条件下半干旱雨养春小麦生育后期旗叶光合的气孔和非气孔限制[J].中国生态农业学报,2015,23(2):174-182. Yang Z S,Zhang Q,Hao X C.Stomatal or non-stomatal limitation of photosynthesis of spring wheat flag leaf at late growth stages under natural conditions in semiarid rainfed regions[J].Chinese Journal of Eco-Agriculture, 2015,23(2): 174-182.(in Chinese) [28]鱼腾飞,冯起,司建华.极端干旱区多枝柽柳叶片气孔导度的环境响应模拟[J].植物生态学报,2012,36(6):483-490. Yu T F,Feng Q,Si J H.Simulating responses of leaf stomatal conductance to environmental factors for Tamarix ramosissma in an extreme arid region of China [J]. Chinese Journal of Plant Ecology,2012, 36(6):483-490.(in Chinese) [29]唐凤德,武耀祥,韩士杰,等.长白山阔叶红松林叶片气孔导度与环境因子的关系[J].生态学报,2008,28(11):5649-5655. Tang F D,Wu Y X,Han S J,et al.Relationship of stamatal conductance of leaf with environmental factors in broadleaved Korean pine forest at Changbai Mountain[J]. Acta EcologicaSinica, 2008,28(11): 5649-5655. (inChinese) [30]王绍武,马树庆,陈莉,等.低温冷害[M].北京:气象出版社,2009:23-40. Wang S W,Ma S Q,Chen L,et al.Low temperature and cold damage[M]. Beijing: China MeteorologicalPress, 2009:23-40. (inChinese)