中国农业气象 ›› 2025, Vol. 46 ›› Issue (4): 546-557.doi: 10.3969/j.issn.1000-6362.2025.04.010

• 农业生物气象栏目 • 上一篇    下一篇

红光背景下不同方式介入蓝光对番茄植株的影响效果

高翔宇，刘艺涵，郭文忠，魏晓明，郑文刚，陈晓丽   

  1. 1.北京市农林科学院智能装备技术研究中心，北京 100097；2.天津农学院园艺园林学院，天津 300384
  • 收稿日期:2024-05-24 出版日期:2025-04-20 发布日期:2025-04-14
  • 作者简介:高翔宇，E-mail：G15166294008@163.com
  • 基金资助:
    北京市智能温室蔬菜创新团队项目（BAIC12−2025）；北京市乡村振兴农业科技课题（NY2501010225）

Impact by Different Methods of Intervening Blue Light to Red Light on Tomato Plants

GAO Xiang-yu , LIU Yi-han , GUO Wen-zhong , WEI Xiao-ming , ZHENG Wen-gang , CHEN Xiao-li   

  1. 1.Intelligent Equipment Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China；2.College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin 300384
  • Received:2024-05-24 Online:2025-04-20 Published:2025-04-14

PDF (PC)

14

摘要：

在全人工光型植物工厂中，以番茄品种‘丰收74−560RZ F1’为材料，以供光模式可调的LED灯为光源进行试验。试验以纯红光R为对照，设置蓝光补充和蓝光取代两种介入方式，蓝光补充介入即在红光背景光基础上，间隔不同时间补充蓝光B，其中蓝光补充强度分别为32、40、64和80μmol·m−2·s−1，相应试验处理分别记作R/RB32、R/RB40、R/RB64和R/RB80。蓝光取代介入即在红光背景光基础上，分别以20μmol·m−2·s−1光强度的蓝光B间歇取代红光，蓝光取代介入时间间隔分别为0、1min、1h和4h，试验处理分别记作RB、R/RB(1min)、R/RB(1h)和R/RB(4h)。在此背景下探究非连续供光模式对番茄生长发育、抗氧化系统及荧光特性的影响。结果表明：（1）与纯红光对照相比，蓝光补充介入处理降低番茄株高9.94%～19.62%，蓝光取代介入处理提高番茄株高12.29%～36.31%。（2）与对照相比，蓝光的两种介入模式均提高了番茄植株地上部生物量。蓝光补充介入模式中，植株地上部生物量随着蓝光光强的增加、补光时长的减少呈增加趋势，其中，蓝光补充处理R/RB80植株地上部生物量最高，干、鲜重较对照分别显著提高115.45%、198.35%（P＜0.05）；蓝光取代介入模式中，R/RB(1min) 处理植株地上部生物量最高，干、鲜重较对照分别显著提高119.35%和152.31 %（P＜0.05），且各处理间无显著差异。（3）R/RB80处理提高了叶片中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）的活性（较对照分别提高14.21%和14.15%），且降低了叶片中活性氧（ROS）含量（较对照降低18.23%）；R/RB(1min)处理下茎、叶中CAT较对照分别提高2.84%、18.35%，而茎、叶中ROS含量分别降低4.64%、20.80%。（4）与连续红光对照相比，两种蓝光介入模式均提高了Ψo［反应中心捕获的激子将电子传递到初级醌受体(QA)以后其他电子受体的概率］、Fv/Fm（最大光化学效率）、Fv/Fo（潜在光化学效率）、ETo/RC（单位反应中心捕获的用于电子传递的能量）等荧光参数，降低了光合系统热耗散（DIo/CS），且蓝光补充处理下荧光参数数值大都高于蓝光取代处理；R/RB80处理下TRo/RC（单位反应中心捕获的用于还原QA的能量）、ETo/CS（单位面积电子传递的量子产额）和ETo/RC数值均为最高，较对照分别提高0.5%、0.6%、2.1%，R/RB（1min）处理下PI abs（叶片光合性能指数）、ETo/RC和Ψo分别较对照提高17.02%、9.53%、5.44%。整体而言，红光背景下蓝光介入不同程度提高了番茄叶片光合系统的电子传递效率，有利于幼苗形态建成以及生物量的增加，其中R/RB80和R/RB（1min）处理是本实验的较优处理。

关键词: 番茄苗期, LED, 生长发育, 抗氧化性, 荧光特性

Abstract:

Tomato was planted in the artificial light plant factory and exposed to different light treatments. Pure red light was as the control, and set up two blue light intervention methods under red background light. One was blue light supplementation intervention with intensity of 32，40，64 and 80 μmol·m−2·s−1 respectively, namely R/RB32, R/RB40, R/RB64 and R/RB80，and the other was blue light substitution intervention with the intervention time intervals of 0, 1min, 1h, and 4h respectively, namely RB, R/RB(1min) R/RB(1h) and R/RB(4h). The growth, antioxidant system and fluorescence characteristics were analyzed to investigate the effects of different light modes of red and blue light on tomato seedling. The result showed that: (1)compared with pure red light, blue light supplementation intervention reduced tomato plant height by 9.94% to 19.62%, but blue light substitution intervention increased tomato plant height by 12.29% to 36.31%. (2)Biomass of tomato seedlings were both increased under two blue light intervention modes, and the shoot biomass was increased with the increase of supplementary blue light intensity. Among which, the highest dry weight and fresh weight were both detected under R/RB80 blue light supplementation intervention treatments, which were enhanced by 115.45% and 198.35% respectively, while the highest dry weight and fresh weight were both observed under R/RB(1min) in blue light substitution intervention treatments, which were enhanced by 119.35% and 152.31% respectively, compared to the control (P＜0.05). (3)The activity of superoxide dismutase (SOD), catalase (CAT) were both increased under R/RB80 treatment, while the content of active oxygen (ROS) was decreased in tomato leaves. R/RB(1min) treatment increased the activity of CAT in stems and leaves by 2.84% and 18.35% respectively, while ROS content was decreased by 4.64% and 20.8% respectively, compared to the control. (4)Blue light intervention modes both improved fluorescence parameters such as Ψo (the probability of excitons captured by the reaction center transferring electrons to the primary quinone acceptor (QA) and then to other electron acceptors), Fv/Fm (maximal photochemical efficiency), Fv/Fo (potential photochemical efficiency), ETo/RC (the energy captured by the unit reaction center for electron transfer), while reduced DIo/CS (thermal dissipation of photosynthetic system). Moreover, the fluorescence parameter values under blue light supplementation mode were mostly higher than those under blue light substitution mode. The values of TRo/RC (the energy captured by the unit reaction center for reducing QA), ETo/CS (quantum yield of electron transfer per unit area), and ETo/RC were the highest under R/RB80 treatment, which were increased by 0.5%, 0.6%, and 2.1% respectively compared to the control. PIabs (leaf photosynthetic performance index), ETo/RC, and Ψo were increased by 17.02%, 9.53%, and 5.44% under R/RB(1min) treatment, respectively, compared to the control. Overall, the electron transfer efficiency of tomato leaf photosynthetic system was improved under blue light intervention with red light background, which was beneficial for seedling morphology and biomass increase. Among which, R/RB80 and R/RB(1min) treatments were the better treatments in this experiment.

Key words: Tomato seedlings, LED, Grow, Antioxidant enzyme, Chlorophyll fluorescence

版权所有 © 《中国农业气象》编辑部
地址：北京中关村南大街12号中国农科院环发所　邮编：100081
电话：010-82109772;82109774　 Email：lliuyuan@caas.cn; zgnyqx@caas.cn
本系统由北京玛格泰克科技发展有限公司设计开发