中国农业气象

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La与UV-B辐射胁迫对大豆叶片细胞叶绿体超微结构的影响

彭倩;周青;叶亚新;   

  1. 江南大学工业生物技术教育部重点实验室,江南大学工业生物技术教育部重点实验室,江南大学工业生物技术教育部重点实验室 无锡214222,无锡214222,无锡214222,苏州科技学院生物学系
  • 出版日期:2008-02-10 发布日期:2008-02-10
  • 基金资助:
    国家自然科学基金(30570323);; 国家发改委稀土专项基金(IFZ20051210)

Influence of La and Ultraviolet-B Stress on Chloroplast Ultrastructure of Soybean Leaves

PENG Qian1,ZHOU Qing1,YE Ya-xin1,2(1.Key Laboratory of Industrial Biotechnology of the Ministry of Education,Southern Yangtze University,Wuxi 214222,China;2.Department of Biology,College of Science and Technology of Suzhou)   

  • Online:2008-02-10 Published:2008-02-10

摘要: 采用水培法研究了稀土La(Ⅲ)对紫外辐射(UV-B:0.15Wm-2,0.45Wm-2)胁迫下大豆(Glycine max)叶片细胞超微结构尤其是对叶绿体结构的影响。结果表明:La(Ⅲ)处理下叶绿体中的类囊体片层排列整齐、有序、清晰;UV-B辐射胁迫下叶绿体内类囊体片层紊乱、膨胀甚至模糊不清,高强度UV-B辐射(T2)对叶绿体的影响大于低强度辐射(T1);而La(Ⅲ)能够影响UV-B辐射胁迫下叶绿体在细胞环境内的空间分布,减轻UV-B辐射对叶绿体外膜的破坏,使叶绿体内类囊体片层变得有序清晰,且对低强度UV-B辐射(T1)的缓解效果优于高强度(T2)。

关键词: La, UV-B辐射, 大豆叶片, 叶绿体, 超微结构

Abstract: Influence of La on chloroplast ultrastructure of soybean(Glycine max) leaves under elevated ultraviolet-B radiation(UV-B,280-320 nm) was studied.The results showed that the thylakoid in the chloroplast was placed in an orderly arrangement and observational clearly,as soybean leaves were pretreated by La(Ⅲ).The UV-B stress led to a chaos arrangement of the thylakoid in the chloroplast,expand or even indiscoverable.The impact on the thylakoid by the high intensity UV-B radiation(T2) was bigger than that by the low intensity UV-B radiation(T1).However,La(Ⅲ) changed the spatial location of chloroplast in the cell under the condition of UV-B stress.Therefore,the destruction of the chloroplast membrane caused by UV-B stress was alleviated.Consequently,the arrangement of the thylakoid in the chloroplast became orderly and clearly.The effect of the alleviation by La(Ⅲ) under the low intensity UV-B radiation(T1) was better than that under the high intensity UV-B radiation(T2).

Key words: Lanthanum(La), Lanthanum(La), Ultraviolet-B radiation, Soybean Leaves, Chloroplast, Ultrastructure