沙尘胁迫对榅桲叶片光合和叶绿素荧光特性的影响

doi:10.3969/j.issn.1000-6362.2018.10.007

摘要/Abstract

摘要： 以榅桲（Cydonia oblonga Mill）为研究材料，以无沙尘覆盖为对照（CK），设置轻度沙尘胁迫（5mg·cm-2）和重度沙尘胁迫（12mg·cm?2）两个处理，并分别在处理第10、20、30和40天时，测定其叶片光合特性和荧光参数的变化。结果表明：沙尘处理下榅桲叶片的净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）均随处理时间的延长呈下降趋势，胞间CO2浓度（Ci）呈先升后降的变化趋势；最大光量子效率（Fv/Fm）在处理第10天显著下降，此后随处理时间的延长逐渐回升至与对照一致水平。最大荧光（Fm）、非光化学淬灭（NPQ_Lss）、PSⅡ潜在活性（Fv/Fo）、光化学反应淬灭（qP_Lss）随处理时间的延长整体均呈上升趋势，初始荧光（Fo）呈下降趋势。说明榅桲在沙尘胁迫处理前期受非气孔因素的影响较大，沙尘胁迫导致榅桲叶片PSⅡ原初光能转换效率较低，对榅桲产生光抑制，导致Pn下降。而在处理后期，榅桲叶片主要通过增加qP_Lss、Fm、NPQ_Lss等来增强PSⅡ电子传递能力，提高非辐射性热耗散来消耗过剩光能，保护光合机构。

关键词: 榅桲, 沙尘, 净光合速率, 胞间CO2浓度, 最大光量子效率

Abstract: Taking Cydonia oblonga Mill as an experimental material to examine the effects of dust on the photosynthesis and chlorophyll fluorescence parameters, three levels of dust treatments during 40 days which comprise non-dust cover（CK）, mild dust treatment （5mg·cm-2）and serious dust treatment（12mg·cm?2）. The results showed that net photosynthetic rate（Pn）, stomatal conductance（Gs）, and transpiration rate（Tr）of the leaves under the dust treatment showed a downward trend with the treatment time. Intercellular CO2 concentration（Ci）showed a trend of increasing first and then decreasing, The maximum light quantum efficiency（Fv/Fm）decreased significantly on the 10th day of treatment, and then gradually increased to the control level with the prolonged treatment time. For two dust treatments, maximal fluorescence（Fm）, non-photochemical quenching under stable state（NPQ_Lss）, potential activity（Fv/Fo）and photochemical quenching under stable state（qP_Lss）increased with treatment time, but minimal fluorescence（Fo）decreased with treatment time .These results indicated that the non-stomatal inhibition had a significant on the photosynthetic rate greater at the beginning, the dust stress reduced the primary photochemical efficiency of PSⅡto cause photoinhibition. In later stage, Cydonia oblonga Mill promoted the efficiencies of photosynthetic electron transport due to increase such as qP_Lss，Fm and NPQ_Lss. On the other hand, Cydonia oblonga Mill increased non photochemical dissipation to protect the photosynthetic organs.

Key words: Cydonia oblonga Mill, Dust stress, Net photosynthetic rate, Intercellular CO2 concentration, Maximum light quantum efficiency

王孟辉，巴特尔·巴克，康丽娟，薛亚荣，萨吉旦·阿卜杜克日木，祖力克艳·麻那甫. 沙尘胁迫对榅桲叶片光合和叶绿素荧光特性的影响[J]. 中国农业气象, 2018, 39(10): 685-692.

WANG Meng-hui，Batur BAKE，KANG Li-juan，XUE Ya-rong，Sajida ABDUKIRIM，Zulkeya MANAP. Effect of Dust Stress on the Photosynthetic and Chlorophyll Fluorescence Characteristics of Cydonia oblonga Mill[J]. Chinese Journal of Agrometeorology, 2018, 39(10): 685-692.

参考文献

[1] 俞德俊.中国植物志[M].北京:科技出版社,1986:344-345. Yu D J.Flora of China[M].Beijing:Science Press,1986:344- 345.(in Chinese) [2] 秦伟,韩晶,克热木·伊力.盐胁迫对榅桲种子萌发率、成苗率和酶活性的影响[J].新疆农业科学,2009,46(1):23-27. Qin W,Han J,Keremu Y.Effects of salt stress on germination percentage,seedling stand percent enzyme activity in Cydonia oblonga Mill Seed[J].Xinjiang Agricultural Sciences,2009, 46(1):23-27.(in Chinese) [3] 王济宪.新疆的稀有果类:榅桲[J].特种经济动植物,2000, (5):30. Wang J X.Rare fruit of Xinjiang:Cydonia oblonga Mill[J]. Special Economic Animal and Plant,2000,(5):30.(in Chinese) [4] 夏扎旦·夏克尔,买合布白,艾克白尔,等.新疆榅桲中的鞣质类化合物及其生物活性[J].生物技术通讯,2006,17(5): 840-842. Xizadam X,Mahbuba,Akbar,et al.Biological activities of tannins in quince (Cydonia oblanga Miller) in Xinjiang[J]. Letters in Biotechnology,2006,17(5):840-842.(in Chinese) [5] 马木提·库尔班.新疆榅桲中总生物碱的测定[J].食品科学,2005,26(2):186-189. Mamut K.Assay on total alkaloid in Xinjiang Cydonia Oblonga Mill[J].Food Science,2005,26(2):186-189.(in Chinese) [6] 裴斌,张光灿,张淑勇,等.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报,2013,33(5): 1386-1396. Pei B,Zhang G G,Zhang S Y,et al.Effects of soil drought stress on photosynthetic characteristics and antioxidant enzyme activities in Hippophae rhamnoides Linn. seedings[J].Acta Ecologica Sinica,2013,33(5):1386-1396.(in Chinese) [7] 帕提古力·麦麦提,巴特尔·巴克,海利力·库尔班.沙尘胁迫对阿月浑子光合作用及叶绿素荧光特性的影响[J].生态学报,2014,34(22):6450-6459. Patigul M,Batur B,Halil K.Influence of dust stress on the photosynthetic and chlorophyll fluorescence characteristics of Pistacia vera L[J].Acta Ecologica Sinica,2014,34(22):6450- 6459.(in Chinese) [8] 祖力克艳·麻那甫,牙库甫江·马合木提,萨吉旦·阿卜杜克日木,等.不同沙尘覆盖对3种果树叶片光合特性及叶绿素荧光特性的影响[J].西南农业学报,2017,30(4):757-761. Zulkeya M,Ablet M,Sajida A,et al.Effects of different amount of dust covering on photosynthetic characteristics of Malus pumila Mill.Armeniaca vulgaris Lam. and Ziziphus jujuba Mill.leaves[J].Southwest China Journal of Agricultural Sciences,2017,30(4):757-761.(in Chinese) [9] 帕提古力·麦麦提.沙尘胁迫对阿月浑子叶片光合生理的影响[D]. 乌鲁木齐:新疆农业大学,2013. Patigul M.The effect of dust stress on the photosynthetic physiology of Pistacia vera L.leaves[D].Urumqi: Xinjiang Agricultural University,2013.(in Chinese) [10] 张曼义,杨再强,侯梦媛.土壤水分胁迫对设施黄瓜叶片光合及抗氧化酶系统的影响[J].中国农业气象,2017,38(1): 21-30. Zhang M Y,Yang Z Q,Hou M Y.Effects of soil water stress on photosynthetic characteristics and antioxidant enzyme system of cucumber leaves in greenhouse[J].Chinese Journal of Agrometeorology,2017,38(1):21-30.(in Chinese) [11] Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982, 33(1):317-345. [12] 侯奇奇,杨再强,史五一,等.淹渍对花椰菜根系活力和叶片光合特性的影响[J].中国农业气象,2016,37(1):51-58. Hou Q Q,Yang Z Q,Shi W Y,et al.Effect of waterlogging stress on root activity and leaf photosynthesis character of cauliflower[J].Chinese Journal of Agrometeorology,2016, 37(1):51-58.(in Chinese) [13] Massacci A,Nabiew S M,Pietrosanti L,et al.Response of photosynthesis apparatus of cotton to the onset of drought stress under field conditions by gas change analysis and chlorophyll fluorescence imaging[J].Plant Physiology and Biochemistry,2008,46(2):189-195. [14] Efeoglu B,Ekmekci Y,Cicek N.Physiological responses of three maize cultivars to drought stress and recovery[J].South Afican Journal of Botany,2009,75(1):34-42. [15] 曲丹阳,张立国,顾万荣,等.壳聚糖对镉胁迫下玉米幼苗根系生长及叶片光合的影响[J].生态学杂志,2017,36(5): 1300-1309. Qu D Y,Zhang L G,Gu W R,et al.Effects of chitosan on root growth and leaf photosynthesis of maize seedlings under cadmium stress[J].Chinese Journal of Ecology,2017,36(5): 1300-1309.(in Chinese) [16] 熊宇,杨再强,薛晓萍,等.遮光处理对温室黄瓜幼龄植株叶片光合参数的影响[J].中国农业气象,2016,37(2):222-230. Xiong Y,Yang Z Q,Xue X P,et al.Effect of shading on photosynthetic parameters in greenhouse cucumber leaves[J]. Chinese Journal of Agrometeorology,2016,37(2):222-230.(in Chinese) [17] 张亚娟,谢忠奎,赵学勇,等.水分胁迫对东方百合光合特性、叶绿素荧光参数及干物质积累的影响[J].中国沙漠,2011,31(4):884-888. Zhang Y J,Xie Z K,Zhao X Y,et al.Effects of water stress on photosynthetic characteristics,chlorophyll fluorescence,and dry matter of oriental lilies[J].Journal of Desert Research, 2011,31(4):884-888.(in Chinese) [18] 熊冬兰,李静,徐胜,等.臭氧胁迫对梓树光合生理特性的影响[J].生态学杂志,2017,36(4):944-950. Xiong D L,Li J,Xu S,et al.Effects of elevated O3 concentration on photosynthetic physiological characteris- tics of Catalpa ovata[J].Chinese Journal of Ecology,2017, 36(4):944-950.(in Chinese) [19] 张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448. Zhang S R.A discussion on chlorophyll fluorescence kinetics parameters and their significance[J].Chinese Bulletin of Botany,1999,16(4):444-448.(in Chinese) [20] 李菊艳,赵成义,闫映宇,等.盐分对胡杨幼苗生长及光合特性的影响[J].中国沙漠,2010,30(1):80-86. Li J Y,Zhao C Y,Yan Y Y,et al.Effects of salt on the growth and photosynthetic characteristics of Populus euphratica seedlings[J].Journal of Desert Research,2010,30(1):80-86. (in Chinese) [21] 王伟,王岩,梁变变,等.初花期喷镧对UV-B辐射增强下紫花苜蓿光合及荧光特性的影响[J].中国农业气象,2017, 38(4):230-239. Wang W,Wang Y,Liang B B,et al.Effect of spraying lanthanum in early flowering stage on photosynthetic characters and chlorophyll fluorescence parameters of Medicago sativa under enhanced UV-B radiation[J]. Chinese Journal of Agrometeorology,2017,38(4):230-239.(in Chinese) [22] 沈亮,陈君,刘赛,等.脱水胁迫和光合日变化对梭梭和白梭梭叶绿素荧光参数的影响[J].应用生态学报,2015,26(8): 2321-2328. Shen L,Chen J,Liu S,et al.Influence of dehydration and diurnal variation on characteristics of chlorophyll fluoresc- ence of leaves in Haloxylon ammodendron and H.persicum [J].Chinese Journal of Applied Ecology,2015,26(8):2321- 2328.(in Chinese) [23] 郭春芳,孙云,唐玉海,等.水分胁迫对茶树叶片叶绿素荧光特性的影响[J].中国生态农业学报,2009,17(3):560-564. Guo C F,Sun Y,Tang Y H,et al.Effect of water stress on chlorophyll fluorescence in leaves of tea plant (Camellia sinensis)[J].Chinese Journal of Eco-Agriculture,2009, 17(3):560-564.(in Chinese) [24] Gilmore A M,Yamamoto H Y.Zeaxanthin formation and energy dependent fluorescence quenching in pea chloroplasts under artificially mediated linear and cyclic electron transport[J].Plant Physiology,1991,96:635-643.