中国农业气象 ›› 2019, Vol. 40 ›› Issue (04): 230-239.doi: 10.3969/j.issn.1000-6362.2019.04.004

• 论文 • 上一篇    下一篇

CO2浓度增高对水稻籽粒淀粉代谢相关酶活性的影响

王惠贞,武荣盛,蔺汝罡,韩雪   

  1. 1.内蒙古生态与农业气象中心,呼和浩特 010051;2.内蒙古自治区气象局,呼和浩特 010051;3.中国农业科学院农业环境与可持续发展研究所,北京 100081
  • 出版日期:2019-04-20 发布日期:2019-04-17
  • 作者简介:王惠贞(1988-),女,工程师,研究方向为农业气象。E-mail: whz0301@163.com
  • 基金资助:

    中国气象局气候变化专项(CSF201606);国家自然科学基金(41505100)

Effect of Elevated CO2 to Starch Metabolizing Enzyme Activities in Grains of Rice

WANG Hui-zhen, WU Rong-sheng, LIN Ru-gang, HAN Xue   

  1. 1. Inner Mongolia Ecological and Agricultural Meteorological Center, Hohhot 010051, China; 2. Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081; 3. Inner Mongolia Meteorological Bureau, Hohhot 010051
  • Online:2019-04-20 Published:2019-04-17

摘要:

以高产优质粳稻松粳9号和稻花香2号为材料,利用开放式空气CO2浓度富集系统(FACE)实验平台,研究CO2浓度增高对水稻籽粒淀粉代谢相关酶活性的影响。试验设正常大气CO2浓度(400±40μmol·mol-1)和高CO2浓度(600±60μmol·mol-1),测定开花后两个水稻品种籽粒中ADPG焦磷酸化酶、淀粉合成酶和淀粉分支酶活性的变化。结果表明,CO2浓度增高对不同灌浆进程中酶活性的影响程度有显著差异,对乳熟期之后ADPG焦磷酸化酶、可溶性和颗粒型淀粉合成酶活性的表达均有较明显的促进作用,仅阻碍了乳熟期籽粒中淀粉分支酶活性的表达;淀粉代谢相关酶活性对CO2浓度增高的响应因品种而异,松粳9号籽粒中ADPG焦磷酸化酶活性受CO2浓度增高的影响较大,而稻花香2号淀粉合成酶活性受其影响更大。说明随着灌浆进程的推进,CO2浓度增高对淀粉生物合成途径中关键酶活性表达的影响程度存在明显的时段特征,且不同品种的响应程度有显著差异,总体来看,CO2浓度增高可在一定程度上促进淀粉代谢相关酶活性的表达。

关键词: CO2浓度, FACE系统, 水稻, ADPG焦磷酸化酶, 淀粉合成酶, 淀粉分支酶

Abstract:

Free-air CO2 Enrichment (FACE) experimental system was conducted to find out the change trend of relevant enzymes activity about grain starch synthesis in atmospheric CO2 concentration, using high yield and good quality japonica varieties Songjing 9 and Daohuaxiang 2. It was treated with two atmospheric CO2 concentrations: elevated carbon dioxide(600±60μmol·mol-1) and ambient carbon dioxide(400±40μmol·mol-1). The ADPG pyrophosphorylase enzyme activity, starch synthase enzyme activity and starch branching enzyme activity in grains of two rice varieties were measured after flowering. The results showed that the effect of elevated CO2 on the enzyme activity during different grain-filling processes was significantly different, which obviously promoted the expression of ADPG pyrophosphorylase enzyme, soluble and granular starch synthase enzyme activity after the milky stage, but only hindered the expression of starch branching enzyme activity in the grain endosperm at milky stage. Meanwhile, response of starch metabolizing enzyme to elevated CO2 had the genotypic difference with different rice varieties. For the activity of ADPG pyrophosphorylase enzyme, Songjing9 was more affected by elevated CO2, while Daohuaxiang2 was more affected by elevated CO2 for the activity of starch synthase enzyme. The conclusion showed that there was an obvious time characteristics about the influence degree of elevated CO2 to expression of the key enzyme activity during the starch biosynthetic pathway, with the advancement of grouting process. Response degree of different varieties rice on elevated CO2 existed significant differences. Overall, atmospheric CO2 concentration could promote the synthesis of starch in a certain extent.

Key words: Elevated CO2, Free-air carbon dioxide enrichment(FACE), Rice, ADPG pyrophosphorylase enzyme, Starch synthase enzyme, Starch branching enzyme