大穗型水稻BL006和R-农白籽粒灌浆过程中源-流-库特性分析

doi:10.3969/j.issn.1000-6362.2019.09.003

摘要/Abstract

摘要： 以大穗型水稻品种BL006和R-农白为试验材料，中穗型品种黄华占为对照，在大田栽培条件下，比较不同穗型水稻品种的灌浆动态、群体干物质积累、茎鞘非结构性碳水化合物（NSC）含量、输导组织特征及产量性状，探讨大穗型水稻品种的灌浆结实特性及源-流-库特性。结果表明：（1）大穗型水稻品种BL006和R-农白灌浆起步早、前期灌浆速率快，最终粒重高，不同部位籽粒的灌浆动态基本一致，部位间互为同步灌浆。（2）与中穗型水稻品种相比，大穗型水稻品种BL006和R-农白的穗粒数和千粒重分别升高16.83%、33.75%和13.19%、10.07%，谷粒充实率无显著差异，每穴有效穗数及R-农白的受精率分别降低39.48%、31.24%和10.78%；大穗型水稻品种BL006（全生育期）和R-农白（齐穗期后）的群体干物质积累量升高36.06%和6.59%；灌浆期间茎鞘NSC含量升高99.03%和70.32%；穗颈维管束数量、面积和枝梗维管束的面积显著增加。总之，大穗型水稻品种BL006和R-农白的灌浆速率高、灌浆耗时短、粒重高，其中，BL006的受精率更高，籽粒灌浆更优。灌浆期间，两个大穗型水稻品种“源”足、“流”畅，通过适当减少有效穗数，实现了籽粒的优异灌浆充实，这为探明大穗型水稻品种的灌浆特性及调控机理奠定了基础。

关键词: 大穗型水稻, 籽粒灌浆, 源-流-库, 群体干物质积累, 有效穗数

Abstract: Field experiment was conducted with two heavy panicle rice cultivars (BL006, R-nongbai) and one medium panicle rice cultivar (Huanghuazhan). Grain filling characteristics, dry matter accumulation, non-structural carbohydrate (NSC) content in stem-sheath, vascular bundle characters and yield traits were investigated to elucidate characteristics of grain filling and source-flow-sink for heavy panicle rice cultivars. Results showed that: (1) heavy panicle rice cultivars showed earlier grain growth, higher grain filling rate at early grain filling stage, heavier grain weight, and synchronous filling dynamic among spikelets on the different positions. (2) Compared with Huanghuazhan, spikelets per panicle and 1000-grain weights of BL006 and R-nongbai were increased by 16.83%, 33.75% and 13.19%, 10.07%, but filled-grain percentages were not significantly influenced, and effective panicles per hill of the two cultivars and spikelet fertility of R-nongbai were significantly decreased by 39.48%, 31.24% and 10.78%, respectively. Dry matter accumulation was increased by 36.06% in BL006 during the growth stage and 6.59% in R-nongbai after full heading, and NSC contents in stem-sheath were increased by 99.03% and 70.32%, and better vascular bundle characters of panicle neck and branches were observed as well. In a conclusion, heavy panicle rice cultivars BL006 and R-nongbai showed higher grain filling rate, shorter grain filling duration and heavier grain weight, and BL006 showed higher spikelet fertility and superior grain filling than R-nongbai. During grain filling, the two heavy panicle rice cultivars had sufficient source and unobstructed flow, and good grain filling was achieved by reducing appropriately effective panicle per hill. It laid the foundation for exploring the characteristics of grain filling and regulation mechanism of heavy panicle rice cultivars.

Key words: Large panicle rice, Grain filling, Source-flow-sink, Dry matter accumulation, Effective panicle

陈建珍，闫浩亮，刘科，朱开典，田小海. 大穗型水稻BL006和R-农白籽粒灌浆过程中源-流-库特性分析[J]. 中国农业气象, 2019, 40(09): 574-582.

CHEN Jian-zhen, YAN Hao-liang, LIU Ke, ZHU Kai-dian, TIAN Xiao-hai. Source-flow-sink Characteristics of Heavy Panicle Rice BL006 and R-nongbai[J]. Chinese Journal of Agrometeorology, 2019, 40(09): 574-582.

参考文献

[1] Normile D.Reinventing rice to feed the world[J].Science, 2008,321(5887):330-333. [2] Kato T,Shinmura D,Taniguchi A.Activities of enzymes for sucrose-starch conversion in developing endosperm of rice and their association with grain filling in extra-heavy panicle types[J].Plant Production Science,2007,10(4):442-450. [3] Peng S B,Cassman K G,Virmani S S,et al.Yield potential trends of tropical rice since the release of IR8 and the challenge of increasing rice yield potential[J].Crop Science, 1999,39(6):1552-1559. [4] Yang J C, Zhang J H.Grain-filling problem in ‘super’ rice [J].Journal of Experimental Botany,2010a,61(1):1. [5] Shiotsu F, Liu J,Toyota M,et al.Studies on varietal difference of ripening ability in rice:effects of the yield contents and the yield capacity on ripening[J].Japanese Journal of Crop Science,2006,75(4):492-501. [6] Kobata T,Yoshida H,Masiko U,et al.Spikelet sterility is associated with a lack of assimilate in high-spikelet-number rice[J].Agronomy Journal,2013,105(6):1821-1831. [7] 周红英,张桂莲,肖应辉,等.超大穗型水稻R1126及其组合穗颈输导组织与籽粒灌浆结实的关系[J].中国水稻科学,2014, 28(4):411-418. Zhou H Y,Zhang G L,Xiao Y H,et al.Relationship between the conducting tissue in the first internode and the grain-filling of super large panicle rice restore line R1126 and its derived combinations[J].Chinese Journal of Rice Science,2014,28(4): 411-418.(in Chinese) [8] Jiang Q,Du Y L,Tian X Y,et al.Effect of panicle nitrogen on grain filling characteristics of high-yielding rice cultivars [J].European Journal Agronnmy,2016,74:185-192. [9] 顾世梁, 朱庆森, 杨建昌,等.不同水稻材料籽粒灌浆特性的分析[J].作物学报,2001,27(1):7-14. Gu S L,Zhu Q S,Yang J C,et al.Analysis on grain filling characteristics for different rice types[J].Acta Agronomica Sinica 2001,27(1):7-14.(in Chinese) [10] Zhang H,Chen T T,Wang Z Q,et al.Involvement of cytokinins in the grain filling of rice under alternate wetting and drying irrigation[J].Journal of Experimental Botany, 2010,61(13):3719-3733. [11] Peng T,LV Q,Zhao Y F,et al.Superior grains determined by grain weight are not fully correlated with the flowering order in rice[J].Journal of Integrative Agriculture,2015,14(5): 847-855. [12] 韦还和,孟天瑶,李超,等.籼粳交超级稻甬优538的穗部特征及籽粒灌浆特性[J].作物学报,2015,41(12):1858-1869. Wei H H,Meng T Y,Li C,et al.Panicle traits and grain-filling characteristics of Japonica/Indica hybrid super rice Yongyou 538[J].Acta Agronomica Sinica,2015,41(12):1858-1869.(in Chinese) [13] 杨建昌.水稻弱势粒灌浆机理与调控途径[J].作物学报, 2010,36(12):2011-2019. Yang J C.Mechanism and regulation in the filling of inferior spikelets of rice[J].Acta Agronomica Sinica,2010,36(12): 2011-2019.(in Chinese) [14] 张彩霞,符冠富,奉保华,等.水稻同化物转运及其对逆境胁迫响应的机理[J].中国农业气象,2018,39(2):73-83. Zhang C X,Fu G F,Feng B H,et al.Mechanisms of assimilation transport in phloem of rice and its response to abiotic stress[J].Chinese Journal of Agrometeorology,2018, 39(2):73-83.(in Chinese) [15] 江晓东,吕润,金志凤,等.浙江地区甬优15直播稻的最佳播期分析[J].中国农业气象,2019,40(1):37-44. Jiang X D,Lv R,Jing Z F,et al.Analyzing on the optimum direct-sowing date for Yongyou15 rice variety in Zhejiang region[J].Chinese Journal of Agrometeorology,2019,40(1): 37-44.(in Chinese) [16] 罗海伟,杨重法,田小海.大穗型水稻品种穗部结构特征分析[J].热带作物学报,2013,34(3):418-423. Luo H W,Yang C F,Tian X H.Analysis of panicle structure characteristics of large panicle rice[J].Chinese Journal of Tropical Crop,2013,34(3):418-423.(in Chinese) [17] Prasad P V V,Boote K J,Jr L H A,et al.Species,ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress[J].Field Crops Research,2006,95(2):398-411. [18] Mohammed A R,Tarpley L.Effects of high night temperature and spikelet position on yield-related parameters of rice (Oryza sativa L.) plants[J].European Journal of Agronomy, 2010,33(2):117-123. [19] 朱庆森,曹显祖,骆亦其.水稻籽粒灌浆的生长分析[J].作物学报,1988,14(3):182-193. Zhu Q S,Cao X Z,Luo Y Q.Growth analysis on the process of grain filling in rice[J].Acta Agronomica Sinica,1988,14 (3):182-193.(in Chinese) [20] 潘俊峰. 氮对水稻茎鞘非结构性碳水化合物积累转运特征的影响及其遗传基础研究[D].武汉:华中农业大学,2010. Pan J F.Effects of nitrogen treatments on characteristics of accumulation and translocation of non-structural carbohydrates in stems and their genetic basis[D].Wuhan: Huangzhong Agricultural University,2010.(in Chinese) [21] Yoshinaga S,Takai T,Arai-Sanoh Y,et al.Varietal differences in sink production and grain-filling ability in recently developed high-yielding rice(Oryza sativa L.) varieties in Japan[J].Field Crops Research,2013,150(15):74-82. [22] 魏颖娟,赵杨,邹应斌.不同穗型超级稻品种籽粒灌浆特性[J].作物学报,2016,42(10):1516-1529. Wei Y J,Zhao Y,Zou Y B.Grain-filling characteristics in super rice with different panicle types[J].Acta Agronomica Sinica,2016,42(10):1516-1529.(in Chinese) [23] Fageria N K.Yield physiology of rice[J].Journal of Plant Nutrition,2007,30(6):843-879. [24] Fu J,Huang Z H,Wang Z Q,et al.Pre-anthesis non-structural carbohydrate reserve in the stem enhances the sink strength of inferior spikelets during grain filling of rice[J].Field Crops Research,2011,123(2):170-182. [25] Yang J C,Peng S B,Zhang Z J,et al.Grain and dry matter yields and partitioning of assimilates in Japonica/Indica hybrid rice[J].Crop Science,2002,42(3):766-772. [26] Li H W,Liu L J,Wang Z Q,et al.Agronomic and physiological performance of high-yielding wheat and rice in the lower reaches of Yangtze River of China[J].Field Crops Research,2012,133:119-129. [27] Kato T.Variation in the branching system of vascular bundles within a panicle among rice extra-heavy panicle types[J].Memoirs of the School of Biology-Oriented Science and Technology of Kinki University,2008,21:1-6.