Effects of Soil Water Stress on Photosynthetic Characteristics and Antioxidant Enzyme System of Cucumber Leaves in Greenhouse

doi:10.3969/j.issn.1000-6362.2017.01.003

Abstract

Abstract: Took cucumber variety of ‘Jinyan4’ as experimental materials, the potting experiment in all growth stages of cucumber under different soil water stresses was conducted in Venlo greenhouse of Nanjing University of Information Science &Technology in autumn and winter of 2015/2016. Soil water treatments were divided into four levels: normal water supply (CK, 70%-80% of field water holding capacity), mild water stress (T1, 60%-70% of field water holding capacity), moderate water stress (T2, 50%-60% of field water holding capacity), and severe water stress (T3, 30%-40% of field water holding capacity). Soil water sensor EM50 was used to monitor soil water content. The photosynthetic characteristics and antioxidant enzyme activities of cucumber leaves were determined at seedling stage, tendril stage, flowering stage and ripening stage. The results showed that photosynthetic pigment contents in different treatments had a declining trend under soil water stresses. Compared with CK, chlorophyll a, chlorophyll b and carotenoids content of severe water stress (T3) treatment was reduced by 47.41%, 41.03% and 34.03%, respectively. Chlorophyll a/b had a minor change in different treatments. Leaf net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), transpiration rate (Tr), stomatal conductance (Gs) declined with increase of soil water stress degree. Meanwhile, stomatal limitation value (Ls) increased. Water Use Efficiency (WUE) of severe water stress (T3) treatment was higher than other treatments. With the prolonging of soil water stress days, the malonaldehyde (MDA) contents had a gradual rise and the activity of superoxide dismutase (SOD) and catalase (CAT) increased firstly and then decreased. Conversely, the activity of peroxidase (POD) showed a decreasing trend firstly and then decreased before an increasing trend. With the aggravation of soil water stress, SOD, POD and CAT activity and MDA contents increased gradually. This study indicated that soil water stress made activities of antioxidant enzymes and MDA contents of cucumber leaf increased, but weaken photosynthesis.

Key words: Antioxidant enzyme activities, Stomatal conductance, Transpiration rate, Stomatal limitation value

ZHANG Man-yi, YANG Zai-qiang,HOU Meng-yuan. Effects of Soil Water Stress on Photosynthetic Characteristics and Antioxidant Enzyme System of Cucumber Leaves in Greenhouse[J]. Chinese Journal of Agrometeorology, 2017, 38(01): 21-30.

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