Effect of Different Oligosaccharides by Spraying on Plant Growth and Quality in Lettuce

doi:10.3969/j.issn.1000-6362.2019.12.005

Abstract

Abstract: Experiments in climate chamber were performed to explore the levels of various components of single and mixed oligosaccharides on growth, yield and performance of lettuce. A short-growing type of lettuce (var. ramosa Hort) and three oligosaccharides, such as cello-oligosaccharides (ZH-A), xylo-oligosaccharides (ZH-B), and chitosan oligosaccharides (ZH-C) were selected. The mixed oligosaccharides (ZH-M) (Mass Ratio, ZH-A:ZH-B:ZH-C=1:1:1) and the conventional polysaccharide sodium alginates (GY-D) were prepared for the experiment. 80mg·L?1 of each was applied 4 times (every 3 days) to lettuce by foliar spraying after three days of planting and the water (CK) was used as a control. Growth characteristics (biomass, leaf area and fluorescence photosynthetic), root phenotypic characteristics (root length, root surface area and root volume) and the quality characteristics (soluble sugar, chlorophyll, Vc, and nitrate contents) of lettuce were measured at the harvest stage (23 days after planting). The results showed that foliage spraying can significantly increase the biomass of lettuce in all single oligosaccharide treatments and the (GY-D) treatment. The cello-oligosaccharide showed the significant increase in root growth and decrease in nitrate content. The chitosan oligosaccharide showed the substantial increase in chlorophyll content, the maximum photosynthetic efficiency (Fv/Fm) and the soluble sugar content. The mixed oligosaccharides (ZH-M) showed the best effects on lettuce growth, such as the above and underground biomass and the quality characters. In the ZH-M treatment, the fresh weight and leaf area index increased by 52.58% and 57.60% at the harvest stage. And, root dry weight, total root lengths, root total volume and root surface area increased by 35.07%, 89.10%, 49.23% and 40.68%, respectively. The content of soluble sugar, chlorophyll, and Vc rose by 25.20%, 21.50% and 12.08% respectively, while the nitrate content dropped by 27.65%. In conclusion, significant differences in interaction and regulation regime were found among the different oligosaccharides on plant physiology and growth. Compared with the single oligosaccharide, the mixed oligosaccharides showed the multiplying influences on plant growth and quality improving.

Key words: Lettuce, Oligosaccharide, Mixed-oligosaccharides, Polysaccharide, Nitrate

HE Jiu-xing, ZHAO Jie-chun, BAI Wen-bo, ZHENG Li, ZHANG Yuan-cheng, YU Meng-meng, Kimoto Hisash, Saito Makoto, LV Guo-hua. Effect of Different Oligosaccharides by Spraying on Plant Growth and Quality in Lettuce[J]. Chinese Journal of Agrometeorology, 2019, 40(12): 783-792.

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