Phospholipase D of Winter Wheat is Involved in Responses to Drought Stress

doi:10.3969/j.issn.1000-6362.2019.04.003

Abstract

Abstract: Phospholipase D (PLD) mediate signaling processes of various hormones and adversities by hydrolyzing phospholipids in cellular membrane and producing phosphotidic acid (PA), a messenger. In order to investigate the roles and pathways of PLD in regulation of the stability of cellular membrane, PEG-6000 was adopted to mimic drought stress, and n-butabol (BA) was applied to inhibit PLD-derived PA. Drought resistance, stability of cellular membrane, antioxidant enzymes of wheat seedlings treated by PEG and PEG added BA were compared. The results showed that inhibition of PLD by BA induced slower seedling growth, lower net photosynthesis rate, higher non-stomatal limitation, higher membrane ion leakage and malonaldehyde (MDA), a peroxidation product, and lower POD activity. The results indicated that PLD played its role in regulating POD activity. Thus, a potential signal transduction pathway under drought stress mediated by PLD was explored: drought stress—activation of PLD—enhancement of POD—impairation of membrane lipids peroxidation damage—improvement of stability of cellular membrane—increasing drought resistance of plants.

Key words: Phospholipase D, Drought, Signal transduction, Peroxidation, Antioxidant enzyme, PLD

WANG Ya-jing, ZHANG Xin-ying, HUANG Gui-rong, FENG Fu, ZHONG Xiu-li. Phospholipase D of Winter Wheat is Involved in Responses to Drought Stress[J]. Chinese Journal of Agrometeorology, 2019, 40(04): 222-229.

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