Abstract: In this paper, the simulating acid rain (pH3.0) pollution was used, Brassica chinensis L., a national agricultural geographical indication product, regarded as the model material. The leaves which were sprayed distilled water regard as the control (CK). One treatment is the leaves were sprayed of pH 3.0 artificial acid rain (SY). Another treatment is the leaves were sprayed with 10mg·L−1 Pyridine-2,3,4,5,6-pentacarboxylate solution and pH3.0 artificial acid rain (WS) in sequence. The root activity, plasma membrane permeability (MP), catalase (CAT) activity, oxygen free radical producing rate and proline (PRO) content were studied under the condition of acid rain stress and pyridine-2,3,4,5,6-pentacarboxylate protection. The results showed that the leaf which sprayed 10mg·L−1 pyridine-2,3,4,5,6-pentacarboxylate solution could significantly improve the root activity and CAT activity, protect the cytoplasm membrane, and effectively reduce the PRO content and oxygen free radical production rate in broccoli under acid rain stress. The comprehensive comparison shows that pyridine-2,3,4,5,6-pentacarboxylate have a positive effect on relieving the physiological damage under the acid rain stress and improving the ability of resisting acid rain.

Key words: Brassica chinensis L., Acid rain stress, Pyridine-2,3,4,5,6-pentacarboxylate, Physical and chemical indexe, Chemical control and disaster reduction