Effects of Water Stress on Growth and Physiological Properties of Millet Seedlings

doi:10.3969/j.issn.1000-6362.2019.08.003

Abstract

Abstract:

In order to study the effects of water stress on the growth, membrane lipid peroxidation and osmotic adjustment of broom corn millet seedlings, the experiment was conducted 5 treatments: severe drought, slight drought, normal irrigation control, slight flooding and severe flooding. ‘Jinshu No. 8’ millet as material was grown in sand culture and irrigated with nutrient solution, and indicators including the morphological indicators, biomass, water content of plant, and the membrane permeability, the contents of photosynthetic pigments, MDA, AsA, proline, soluble sugar and soluble protein in leaves were determined on 20th day after treatment. The results showed that the millet seedling grew best under normal irrigation condition, with the largest values of plant height, stem diameter, nodule number, leaf number, maximum leaf area, fresh mass and dry mass of root, stem and leaf, and panicle. All morphological indicators and biomasses were obviously decreased under drought and flooding treatments, and the extent of decrease under severe drought and severe flooding were more obvious than those under slight drought and slight flooding. The water contents of root, stem and leaf, panicle were clearly decreased under drought, but showed different tendency under flooding treatments. The contents of photosynthetic pigments were significantly decreased under drought, while relatively stable under flooding. The membrane permeability, the contents of MDA, AsA and proline were obviously increased under drought and flooding, and the extents of increase under severe drought and severe flooding were more obvious than those under slight drought and slight flooding. The soluble sugar content was clearly increased under drought and was clearly decreased under flooding, the soluble protein content was significantly reduced under drought and was relatively stable under flooding. The research illustrated that the drought and flooding caused the peroxidation injury to the millet seedlings, and the contents of antioxidants and osmotic adjusting materials were increased. But the increase of antioxidants could not completely eliminate the peroxidation injury caused by stress. In addition, the photosynthetic ability was decreased under stress, which caused the inhibition of the growth of millet seedlings. Under the experimental condition, drought stress caused more serious damage to millet seedlings than flooding.

Key words: Millet, Water stress, Growth, Lipid peroxidation, Osmotic adjustment substances

HAN Zhi-ping, ZHANG Hai-xia, ZHANG Xun, LIANG Yan-hua. Effects of Water Stress on Growth and Physiological Properties of Millet Seedlings[J]. Chinese Journal of Agrometeorology, 2019, 40(08): 502-511.

References

[1] 蔡庆生.植物生理学[M].北京:中国农业大学出版社,2014: 306-313. Cai Q S.Plant physiology[M].Beijing:China Agricultural University Press,2014:306-313.(in Chinese) [2] 董杰,贾学峰.全球气候变化对中国自然灾害的可能影响[J].聊城大学学报,2004,17(2):58-62. Dong J,Jia X F.Possible impacts of global climate change on natural disasters of China[J].Journal of Liaocheng University, 2004,17(2):58-62.(in Chinese) [3] 王纶,王星玉,温琪汾,等.中国黍稷种质资源研究与利用[J].植物遗传资源学报,2005,6(4):474-477. Wang L,Wang X Y,Wen Q F,et al.Research and utilization of prosomillet germplasm resource in China[J].Journal of Plant Genetic Resources,2005,6(4):474-477.(in Chinese) [4] 穆志新,郝晓鹏,秦慧彬,等.山西省干旱地区农作物种质资源普查与分析[J].植物遗传资源学报,2016,17(4):637-648. Mu Z X,Hao X P,Qing H B,et al.General survey and analysis of crop germplasm resources in drought area of Shanxi province[J].Journal of Plant Genetic Resources,2016, 17(4): 637-648.(in Chinese) [5] 周晋红.山西省干旱时空分布特征及形成机理研究[D].南京:南京信息工程大学,2010. Zhou J H.Study on temporal and spatial distribution feature of drought and its formation mechanism in Shanxi province [D]. Nanjing:Nanjing University of Information Science & Technology,2010.(in Chinese) [6] 庞鑫.山西省农业干旱时空变化特征及其与气象因子的响应研究[D].太原:太原理工大学,2017. Pang X.Spatial and temporal variation characteristics of agricultural drought and its response to meteorological factors in Shanxi province[D].Taiyuan:Taiyuan University of Technology, 2017.(in Chinese) [7] 袁瑞强,龙西亭,王鹏,等.山西省降水量时空变化及预测[J].自然资源学报,2015,30(4):651-663.? Yuan R Q,Long X T,Wang P,et al.Tempo-spatial variation and forecast of precipitation in Shanxi province[J].Journal of Natural Resources,2015,30(4):651-663.(in Chinese) [8] 张美俊,?杨武德,?乔治军,?等.不同糜子品种萌发期对干旱胁迫的响应及抗旱性评价[J].草地学报,2013,21(2): 302-307. Zhang M J,Yang W D,Qiao Z J,et al.Resistance evaluation and response of 16 millet varieties at germination stage to drought stress[J].Acta Agrestia Sinaca,2013,21(2):302-307. (in Chinese) [9] 王纶,温琪汾,曹厉萍,等.黍稷抗旱种质筛选及抗旱机理研究[J].山西农业科学,2007,35(4):31-34. Wang L,Wen Q F,Cao L P,et al.Drought-resistant germplasm screening and drought-resistance mechanism in proso millet[J].Journal of Shanxi Agricultural Sciences,2007,35(4): 31-34.(in Chinese) [10] 张永清,苗果园.不同施肥水平下黍子根系对干旱胁迫的反应[J].作物学报,2006,32(4):601-606. Zhang Y Q,Miao G Y.The biological response of broom corn millet root to drought stress with different fertilization levels[J].Acta Agronomica Sinica,2006,32(4):601-606.(in Chinese) [11] 冯晓敏.不同黍稷品种耐旱性差异及生理生态特性研究[D].临汾:山西师范大学,2012. Feng X M.Study?on drought tolerance diversity and physiological characteristics of different prosoes[D].Linfen: Shanxi Normal University,2012.(in Chinese) [12] 闫江艳,张永清,冯晓敏,等.干旱胁迫及复水对不同黍稷品种根系生理特性的影响[J].西北植物学报,2012,32(2): 348-354. Yan J Y,Zhang Y Q,Feng X M,et al.Effect of drought stress and rewatering on physiological characteristics of roots in different prosomillet varieties[J].Acta Bot Boreal-Occident Sin,2012,32(2):348-354.(in Chinese) [13] 王素平,郭世荣,胡晓辉,等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响[J].江西农业大学学报,2006,28(2):32-38. Wang S P,Guo S R,Hu X H,et al.Effects of NaCl stress on the content of photosynthetic pigments in the leaves of cucumber(Cucumis sativus L.)seedlings[J].Acta Agriculturae Universitatis Jiangxiensis,2006,28(2):32-38.(in Chinese) [14] Madhava R K V,Sresty T V S.Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses[J].Plant Science,2000,157: 113-128. [15] 汤绍虎,罗充.植物生理学实验教程[M].重庆:西南师范大学出版社,2012:199-202. Tang S H,Luo C.Plant physiology experiment course[M]. Chongqing:Southwest Normal University Press, 2012: 199-202. (in Chinese) [16] 中科院上海植物生理研究所、上海市植物生理学会.现代植物生理学实验指南[M].北京:科学出版社,1999:315-316. Shanghai Institute of Plant Physiology,Chinese Academy of Sciences,Shanghai Society of Plant Physiology.Guidelines for modern plant physiology experiments[M].Beijing: Science Press,1999:315-316.(in Chinese) [17] 李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2002:195-197,258-260. Li H S.Principle and technology of plant physiological and biochemical experiments[M].Beijing:Higher Education Press, 2002:195-197,258-260.(in Chinese) [18] 王孝平,邢树礼.考马斯亮蓝法测定蛋白质含量的研究[J].天津化工,2009,23(3):88-90. Wang X P,Xing S L.Determination of protein quantitation using the method of coomassie brilliant blue[J].Tianjin Chemical Industry,2009,23(3):88-90.(in Chinese) [19] 闫瑞,钱春.无患子幼苗对水分胁迫的生理响应[J].西南大学学报(自然科学版),2014,36(4):29-33. Yan R,Qian C.Physiological response of Sapindus mukorossi Gaertn.seedlings to water stress[J].Journal of Southwest University (Nat Sci Ed),2014,36(4):29-33.(in Chinese) [20] 王三根,宗学凤.植物抗性生物学[M].重庆:西南师范大学出版社,2015:123-140. Wang S G,Zong X F.Plant resistance biology[M].Chongqing: Southwest Normal University Press,2015:123-140.(in Chinese) [21] 武维华.植物生理学(第三版)[M].北京:科学出版社,2018: 110-119,385-391. Wu W H.Plant physiology(3rd Ed)[M].Beijing:Science Press, 2018:110-119,385-391.(in Chinese) [22] Sharma M,Gupta S K,Majumder B,et al.Salicylic acid mediated growth,physiological and proteomic responses in two wheat varieties under drought stress[J].Journal of Proteomics,2017,163:28-51. [23] 朱雨晴,杨再强.不同品种葡萄叶片光合特性对干旱胁迫的响应及旱后恢复过程[J].中国农业气象,2018,39(11): 739-750. Zhu Y Q,Yang Z Q.Photosynthetic responses of different grape cultivars to drought stress and their recovery after drought[J].Chinese Journal of Agrometeorology,2018,39 (11):739-750.(in Chinese) [24] 张立军,刘新.植物生理学(第二版)[M].北京:科学出版社,2011:307-315. Zhang L J,Liu X.Plant physiology(2nd Ed)[M].Beijing: Science Press,2011:307-315.(in Chinese) [25] 裴荣信.黍子的高产抗灾优势[J].山西农业,1998,(6):9-10. Pei R X.High yield and disaster resistance advantages of millet[J].Shanxi Agriculture,1998,(6):9-10.(in Chinese) [26] Nijs I,Ferris R,Blum H.Stomatal regulation in a changing climate:field study using free air temperature increase(FATI) and free air CO2 enrichment[J].Plant Cell and Environment, 1997,42:1041-1050. [27] 王嘉楠,李小艳,魏石美,等.5-ALA对干旱胁迫下小麦幼苗光合作用及D1蛋白的调节作用[J].作物杂志,2018,(5): 121-126. Wang J N,Li X Y,Wei S M,et al.Regulation of exogenous 5-aminolevulinic acid on photosynthesis and D1 protein of wheat seedlings under drought stress[J].Crops,2018,(5): 121-126. (in Chinese) [28] Lawlor D W,Cornic G.Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants[J].Plant,Cell and Environment,2002,25:275-294. [29] 韩瑞宏,卢欣石,高桂娟,等.紫花苜蓿(Medicago sativa)对干旱胁迫的光合生理响应[J].生态学报,2007,27(12): 5230-5236. Han R H,Lu X S,Gao G J,et al.Photosynthetic physiological response of alfalfa (Medicago sativa) to drought stress[J]. Acta Ecologica Sinica,2007,27(12):5230-5236.(in Chinese) [30] 郑海,陈小华,黎健龙,等.水分胁迫对皇竹草形态及叶片光合特性的影响[J].广东农业科学,2014,(13):33-36. Zheng H,Chen X H,Li J L,et al.Effects of water stress on morphology and photosynthetic characteristics of hybrid giant napier(Pennisetum hydridum)[J].Guangdong Agricultural Sciences,2014,(13):33-36.(in Chinese) [31] Zhang H T,Li J J,Yoo J H,et al.Rice Chlorina-1 and Chlorina-9 encode ChlD and ChlI subunits of Mg-chelatase, a key enzyme for chlorophyll synthesis and chloroplast development[J].Plant Molecular Biology,2006,62(3):325-337. [32] 李倩,王明,王雯雯,等.华山新麦草光合特性对干旱胁迫的响应[J].生态学报,2013,32(13):4278-4284. Li Q,Wang M,Wang W W,et al.Response of photosynthetic characteristic of Psathyrostachys Huashanica Keng to drought stress[J].Acta Ecologica Sinica,2013,32(13): 4278-4284.(in Chinese) [33] Ghotbi-Ravandi A A,Shahbazi M,Shariati M,et al.Effects of mild and severe drought stress on photosynthetic efficiency in tolerant and susceptible barley(Hordeum vulgare L) genotypes[J].Journal of Agronomy and Crop Science,2014, 200(6):403-415. [34] 张曼义,杨再强,侯梦媛.土壤水分胁迫对设施黄瓜叶片光合及抗氧化酶系统的影响[J].中国农业气象,2017,38(1): 21-30. Zhang M Y,Yang Z Q,Hou M Y.Effects of soil water stress on photosynthetic characteristics and antioxidant enzyme system of cucumber leaves in greenhouse[J].Chinese Journal of Agrometeorology,2017,38(1):21-30.(in Chinese) [35] 阎秀峰,李晶,祖元刚.干旱胁迫对红松幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,1999,19(6):850-854. Yan X F,Li J,Zu Y G.Effect of drought stress on activity of cell defense enzymes and lipid peroxidation in Korean pine seedlings[J].Acta Ecologica Sinica,1999,19(6):850-854.(in Chinese) [36] 刘旻霞,马建组.逆境胁迫下加工番茄种子萌发及抗氧化酶系统的影响[J].石河子大学学报(自然科学版),2010,28 (4): 422-426. Liu M X,Ma J Z.Effects of stress on germination and antioxidant enzyme system of processing tomato seeds[J]. Journal of Shihezi University (Nat.Sci.Ed.),2010,28(4): 422-426.(in Chinese) [37] 刘佳,郁继华,徐秉良,等.干旱气候条件下水分胁迫对辣椒叶片生理特性的影响[J].核农学报,2012,26(8):1197-1203. Liu J,Yu J H,Xu B L,et al.Effects of water stress on the physiological characteristics of pepper leaves in arid climates[J].Journal of Nuclear Agricultural Sciences,2012, 26(8):1197-1203.(in Chinese) [38] 杨再强,刘朝霞,韩秀君,等.水分胁迫对番茄保护酶活性及果实产量的影响[J].东北农业大学学报,2014,45(3):40-45. Yang Z Q,Liu Z X,Han X J,et al.Effects of water stress on protective enzyme activity and yield of tomato[J].Journal of Northeast Agricultural University,2014,45(3):40-45.(in Chinese) [39] 张旭东,王智威,韩清芳,等.玉米早期根系构型及其生理特性对土壤水分的响应[J].生态学报,2016,36(10):2969-2977. Zhang X D,Wang Z W,Han Q F,et al.Effects of water stress on the root structure and physiological characteristics of early-stage maize[J].Acta Ecologica Sinica,2016,36(10): 2969-2977.(in Chinese) [40] 赵坤.干旱胁迫条件下春大豆生理生化特性研究[D].哈尔滨:东北农业大学,2011. Zhao K.Research on physiological and biochemical characteristics of spring soybean under drought stress [D]. Harbin:Northeast Agricultural University,2011.(in Chinese) [41] Azymi S,Sofalian O,Jahanbakhsh G S,et al.Effect of chilling stress on soluble protein,sugar and proline accumulation in cotton (Gossypium hirsutum L.) genotypes[J].Intl J Agri Crop Sci,2011,4:825-830. [42] 李钱峰,鲁军,余佳雯,等.油菜素内酯与脱落酸互作调控植物生长与抗逆的分子机制研究进展[J].植物生理学报, 2018,54(3):370-378. Li Q F,Lu J,Yu J W,et al.Advances in molecular mechanisms of brassinosteroid-abscisic acid crosstalk coordinating plant growth and stress tolerances[J].Plant Physiology Journal, 2018,54(3):370-378.(in Chinese) [43] Parida A K,Das A B.Salt tolerance and salinity effects on plants:a review[J].Ecotoxicology and Environmental Safety, 2005,60:324-349. [44] 刘景辉,赵海超,任永峰,等.土壤水分胁迫对燕麦叶片渗透调节物质含量的影响[J].西北植物学报,2009,29(7): 1432-1436. Liu J H,Zhao H C,Ren Y F,et al.Change of osmotica in oat leaf under soil moisture stress[J].Acta Bot Boreal-Occident Sin,2009,29(7):1432-1436.(in Chinese) [45] 张保青,杨丽涛,李杨瑞.自然条件下甘蔗品种抗寒生理生化特性的比较[J].作物学报,2011,37(3):496-505. Zhang B Q,Yang L T,Li Y R.Comparison of physiological and biochemical characteristics related to cold resistance in sugarcane under field conditions[J].Acta Agronomica Sinica,2011,37(3):496-505.(in Chinese) [46] Sonja D,Pignocchi C,Noctor G,et al.Low ascorbic acid in the vtc-1 mutant of arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system[J].Plant Physiology,2001,127:426-435. [47] 杜磊,赵尊练,巩振辉,等.水分胁迫对线辣椒叶片渗透调节作用的影响[J].干旱地区农业研究,2010,28(3):188-198. Du L,Zhao Z L,Gong Z H,et al.Effects of water stress on the osmotic regulation of pepper leaves[J].Agricultural Research in Arid Regions,2010,28(3):188-198.(in Chinese) [48] 张巍巍,郑飞翔,王效科,等.臭氧对水稻根系活力、可溶性蛋白含量与抗氧化系统的影响[J].植物生态学报,2009, 33(3):425-432. Zhang W W,Zheng F X,Wang X K,et al.Effects of ozone on root viability,soluble protein content and antioxidant system in rice[J].Chinese Journal of Plant Ecology,2009,33(3): 425-432.(in Chinese) [49] 王琳,杨再强,王明田,等.空气相对湿度对高温下番茄幼苗营养物质含量及干物质分配的影响[J].中国农业气象, 2018,39(5):304-313. Wang L,Yang Z Q,Wang M T,et al.Effect of air humidity on nutrient content and dry matter distribution of tomato seedlings under high temperature[J].Chinese Journal of Agrometeorology,2018,39(5):304-313.(in Chinese)