持续寡照对冬季日光温室黄瓜生长及抗氧化酶活性的影响

doi:10.3969/j.issn.1000-6362.2017.09.001

摘要/Abstract

摘要： 选取黄瓜品种‘德瑞特L108’（Cucumis sativus L. cv. Derit L108）为试材，在日光温室中利用遮阳网设计不同寡照（PAR＜200μmol·m-2·s-1）持续日数（1、3、5、7、9d）及正常光照条件下的恢复试验，观测黄瓜植株的生长指标以及叶片中抗氧化酶活性的变化。结果表明：（1）持续寡照使黄瓜叶片SPAD（相对叶绿素）值增加，净光合速率下降，持续寡照日数越长，黄瓜叶片净光合速率越小，光合恢复能力越差。（2）寡照7d以上对黄瓜植株的形态指标影响显著，株高、茎粗及单株叶面积均低于CK，且经过9d正常光照恢复后，以上指标均无法恢复到正常水平；且持续寡照使黄瓜果实产量和商品性均下降。（3）寡照胁迫下黄瓜叶片中SOD（超氧化物歧化酶）、POD（过氧化物酶）活性增加，CAT（过氧化氢酶）活性先增加后减小，MDA（丙二醛）含量持续增加，持续寡照日数越长，黄瓜叶片受膜脂过氧化损伤越严重，持续寡照7d以上，黄瓜叶片代谢功能难以恢复至正常水平。

关键词: 寡照, 日光温室, 黄瓜, 形态指标, 产量, 酶活性

Abstract: In order to investigate the effects of overcast weather on cucumber (Cucumis sativus L. cv. Derit L108) growth in glasshouse, the experiment was conducted under two levels of photosynthetic active radiation(PAR): normal irradiation and low irradiation (＜200μmol·m-2·s-1 photon), respectively. Five durations of continuous low irradiation were designed (1, 3, 5, 7 and 9 days), then both groups of plants were transferred under normal irradiation to recover for 9 days. The growth index and the activity of antioxidant enzymes in cucumber leaves were observed. The results showed that: (1) the SPAD value in cucumber leaves increased while the net photosynthesis rate decreased under low irradiation. The longer the duration, the smaller the net photosynthetic rate in cucumber leaves, and the worse on the recovery ability in cucumber leaves. (2) The morphological index of cucumber were significantly affected by low irradiation, and the height, the stem diameter and the leaf-area per plant were smaller under low irradiation after 7 and 9 days than the control respectively, and they could not return to the control level after 9 days under normal irradiation. The cucumber yield and the commercial character decreased with the continuous low irradiation. The activities of SOD, POD were increased and the CAT activity increased at the beginning and then decreased while the content of MDA continuous increased. The longer the duration of low irradiation, the cucumber suffered more by membrane lipid peroxidation. The metabolic function of cucumber leaves was difficult to recover when continuous low irradiation for more than 7 days.

Key words: Overcast weather, Glasshouse, Cucumber, Morphological index, Yield, Enzyme activities

熊宇，刁家敏，薛晓萍，吕学梅，张继波. 持续寡照对冬季日光温室黄瓜生长及抗氧化酶活性的影响[J]. 中国农业气象, 2017, 38(09): 537-547.

XIONG Yu, DIAO Jia-min, XUE Xiao-ping, LV Xue-mei, ZHANG Ji-bo. Effects of Continuous Overcast Weather on Cucumber Growth and Antioxidant Enzyme Activities in Glasshouse[J]. Chinese Journal of Agrometeorology, 2017, 38(09): 537-547.

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