Isolation and Identification of Potassium-Solubilizing Bacteria from Rhizosphere Soil of Apple Tree

doi:10.3969/j.issn.1000-6362.2017.11.006

Abstract

Abstract:

In order to detect the population properties and potassium-dissolving characteristics of potassium-dissolving bacteria existing in apple rhizosphere soil, the highly efficient potassium-dissolving bacterial isolates were gained from the rhizosphere soil of apple trees. 118 strains with the ability of potassium-dissolving were isolated with potassium feldspar as the sole potassium source, then the strains were clustered into 29 populations by analysis of repetitive-element PCR (rep-PCR) genomic DNA fingerprinting. Flame spectrophotometer was used to determine the potassium-dissolving capacity, and 5 highly efficient potassium-dissolving bacteria was obtained, whose average soluble potassium content of culture medium reached to 41.47mg·L^-1. K105 performed the highest potassium- dissolving ability, and the available potassium increased by 23.09%. The available potassium increased after being digested by H₂O₂ solution, the maximum was up to 31.22%. After identifying the isolated 5 strains of efficient potassium-dissolving bacteria by bacterial morphology, physiology and biochemical characteristics, and phylogenetic analysis based on 16S rRNA gene sequences, 5 strains were classified into 4 bacterial genera. K1 belongs to Acinetobacter sp., K98, K105 and K115 belong to Pseudomonas sp., and K168 belongs to Bacillus sp.. It provided basis for exploration of new efficient potassium-solubilizing bacteria, contributing to improve apple replanted soil and develop microbial fertilizer special for apple trees.

Key words: Potassium-solubilizing bacteria, Rep-PCR DNA fingerprinting, Isolation, Identification

JIANG Ji-hang, PENG Xia-wei, YAN Zhen-xin, HE Bu-wei, ZHU Chang-xiong, GUO Hui, GENG Bing. Isolation and Identification of Potassium-Solubilizing Bacteria from Rhizosphere Soil of Apple Tree[J]. Chinese Journal of Agrometeorology, 2017, 38(11): 738-748.

References

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