Effect of Elevated CO2 to Starch Metabolizing Enzyme Activities in Grains of Rice

doi:10.3969/j.issn.1000-6362.2019.04.004

Abstract

Abstract:

Free-air CO₂ Enrichment (FACE) experimental system was conducted to find out the change trend of relevant enzymes activity about grain starch synthesis in atmospheric CO₂ concentration, using high yield and good quality japonica varieties Songjing 9 and Daohuaxiang 2. It was treated with two atmospheric CO₂concentrations: elevated carbon dioxide(600±60μmol·mol^-1) and ambient carbon dioxide(400±40μmol·mol^-1). The ADPG pyrophosphorylase enzyme activity, starch synthase enzyme activity and starch branching enzyme activity in grains of two rice varieties were measured after flowering. The results showed that the effect of elevated CO₂ on the enzyme activity during different grain-filling processes was significantly different, which obviously promoted the expression of ADPG pyrophosphorylase enzyme, soluble and granular starch synthase enzyme activity after the milky stage, but only hindered the expression of starch branching enzyme activity in the grain endosperm at milky stage. Meanwhile, response of starch metabolizing enzyme to elevated CO₂ had the genotypic difference with different rice varieties. For the activity of ADPG pyrophosphorylase enzyme, Songjing9 was more affected by elevated CO₂, while Daohuaxiang2 was more affected by elevated CO₂ for the activity of starch synthase enzyme. The conclusion showed that there was an obvious time characteristics about the influence degree of elevated CO₂ to expression of the key enzyme activity during the starch biosynthetic pathway, with the advancement of grouting process. Response degree of different varieties rice on elevated CO₂ existed significant differences. Overall, atmospheric CO₂ concentration could promote the synthesis of starch in a certain extent.

Key words: Elevated CO₂, Free-air carbon dioxide enrichment(FACE), Rice, ADPG pyrophosphorylase enzyme, Starch synthase enzyme, Starch branching enzyme

WANG Hui-zhen, WU Rong-sheng, LIN Ru-gang, HAN Xue. Effect of Elevated CO2 to Starch Metabolizing Enzyme Activities in Grains of Rice[J]. Chinese Journal of Agrometeorology, 2019, 40(04): 230-239.

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