Regulating Effect of Air Humidity on Tomato Growth and Root Exudates during Flowering Period under High Temperature Condition

doi:10.3969/j.issn.1000-6362.2020.09.002

Abstract

Abstract: Increasing air humidity to relieve high temperature stress is one of the common measures in greenhouse management. To study the regulation mechanism of air relative humidity on tomato growth and disaster recovery in high temperature conditions, the tomato variety "Jinguan 5" was used as the experimental material. This experiment was carried out at the Agricultural Experimental Station of Nanjing University of Information Science and Technology from September 2018 to January 2019. Two dynamic temperature (T) levels (daily maximum temperature/daily minimum temperature such as 32℃/22℃ and 38℃/28℃), three relative humidity (RH) levels (50%±5pp, 70%±5pp and 90%±5pp) and four stress days (3d, 6d, 9d and 12d) were set, and 25℃/15℃ and 50%±5pp were taken as control (CK). The net photosynthetic rate (Pn), total dry weigh t(Wtotal), root dry weight (Wroot), root activity (Rv), and the type and concentration of low molecular weight organic acids(LMWOAs) in root soil were measured at 0, 7, 14, 21 and 28d after treatment. The results showed that: (1) Pn and RV decreased, but root shoot ratio and LMWOAs increased significantly under high temperature, and each index value under 32℃ treatment was significantly higher than 38℃, RH increased to 70%, Pn, Wroot and root shoot ratio increased significantly. (2) At flowering stage, tomato mainly secreted oxalic acid and succinic acid. At high temperature, oxalic acid, malic acid, lactic acid, acetic acid and propionic acid increased, while tartaric acid and formic acid decreased. (3) T and RH were negatively correlated with LMWOAs, and Pn, Wroot, Wtotal and Rv were significantly positively correlated with LMWOAs. (4) Increasing RH to 70% at high temperature condition could increase Pn and Wroot and promote the root system to secrete LMWOAs, which was conducive to the recovery and growth of tomato after disaster. However, when RH reached to 90%, Pn and Wroot would be reduced, which will aggravate the damage of high temperature to plants and is not conducive to the recovery after disaster. Therefore, about 70% air humidity can alleviate the high temperature disaster to a certain extent.

Key words: High temperature and high humidity, Tomato, Photosynthesis, Low molecular weight organic acids, Recovery

CLC Number:

XU Chao，YANG Zai-qiang，WANG Ming-tian，HAN Wei，WEI Ting-ting. Regulating Effect of Air Humidity on Tomato Growth and Root Exudates during Flowering Period under High Temperature Condition[J]. Chinese Journal of Agrometeorology, 2020, 41(09): 552-563.

References 37

[1]	Holder R,Cockshull K E.Effects of humidity on the growth and yield of glasshouse tomatoes[J].Journal of Pomology and Horticultural Science,1990,65(1): 31-39.
[2]	王琳,杨再强,王明田,等.空气相对湿度对高温下番茄幼苗营养物质含量及干物质分配的影响[J].中国农业气象,2018,39 (5):304-313.
	Wang L,Yang Z Q,Wang M T,et al.Effect of air humidity on nutrient content and dry matter distribution of tomato seedlings under high temperature[J].Chinese Journal of Agrometeorology,2018,39(5):304-313.(in Chinese)
[3]	Falah M A F,Wajima T,Yasutake D,et al.Responses of root uptake to high temperature of tomato plants (Lycopersicon esculentum Mill.)in soil-less culture[J].Journal of Agricultural Technology,2010,6(3):543-558.
[4]	袁昌洪,杨再强,赵和丽.番茄高温高湿胁迫后的补偿生长[J].生态学杂志,2020,39(2):487-496.
	Yuan C H,Yang Z Q,Zhao H L.Compensatory growth of tomato after high temperature and high humidity stress[J]. Chinese Journal of Ecology,2020,39(2):487-496.(in Chinese)
[5]	韩玮,孙晨曦,赵和丽,等.高温胁迫后小白菜的补偿生长能力及防御机制[J].中国农业气象,2018,39(2):119-128.
	Han W,Sun C X,Zhao H L,et al.Compensatory ability and defense mechanism of Chinese cabbage under high temperature stress[J].Chinese Journal of Agrometeorology, 2018,39(2):119-128.(in Chinese)
[6]	张中典,张大龙,李建明,等.黄瓜气孔导度、水力导度的环境响应及其调控蒸腾效应[J].农业机械学报,2016,47(6): 139-147.
	Zhang Z D,Zhang D L,Li J M,et al.Environmental response of stomatal and hydraulic conductances and their effects on regulating transpiration of cucumber[J].Transactions of the Chinese Society for Agricultural Machinery,2016,47(6):139- 147.(in Chinese)
[7]	杨世琼,杨再强,王琳,等.高温高湿交互对设施番茄叶片光合特性的影响[J].生态学杂志,2018,37(1):57-63.
	Yang S Q,Yang Z Q,Wang L,et al.Effects of high temperature and high humidity interaction on photosynthetic characteristics of greenhouse tomato leaves[J].Journal of Ecology,2018,37(1): 57-63.(in Chinese)
[8]	王琳,杨再强,杨世琼,等.高温与不同空气湿度交互对设施番茄苗生长及衰老特性的影响[J].中国农业气象,2017,38(12): 761-770.
	Wang L,Yang Z Q,Yang S Q,et al.Effects of high temperature and different air humidity on growth and senescence characteristics of greenhouse tomato seedlings[J].Chinese Journal of Agrometeorology,2017,38(12):761-770.(in Chinese)
[9]	薛义霞,李亚灵,温祥珍.空气湿度对高温下番茄光合作用及坐果率的影响[J].园艺学报,2010,37(3):397-404.
	Xue Y X,Li Y L,Wen X Z.Effects of air humidity on the photosynthesis and fruit-set of tomato under high temperature [J].Acta Horticulturae Sinica,2010,37(3):397-404. (in Chinese)
[10]	王德福,段洪浪,黄国敏,等.高温和干旱胁迫对西红柿幼苗生长、养分含量及元素利用效率的影响[J].生态学报,2019, 39(9):3199-3209.
	Wang D F,Duan H L,Huang G M,et al.Effects of high temperature and drought stress on growth,nutrient concentration,and nutrient use efficiency of tomato seedlings[J].Acta Ecologica Sinica,2019,39(9):3199-3209. (in Chinese)
[11]	Finzi A C,Abramoff R Z,Spiller K S,et al.Rhizosphere processes are quantitatively important components of terrestrial carbon and nutrient cycles[J].Global Change Biology,2015,21(5):2082-2094.
[12]	Dakora F D,Phillips D A.Root exudates as mediators of mineral acquisition in low-nutrient environments[M]//Food security in nutrient-stressed environments: exploiting plants’ genetic capabilities.Springer,Dordrecht,2002:201-213.
[13]	Bais H P,Weir T L,Perry L G,et al.The role of root exudates in rhizosphere interactions with plants and other organisms [J].Annual Review of Plant Biology,2006,57(1): 233-266.
[14]	Strobel B W.Influence of vegetation on low-molecular- weight carboxylic acids in soil solution:a review[J]. Geoderma,2001,99(3-4):169-198.
[15]	Pandey R,Meena S K,Krishnapriya V,et al.Root carboxylate exudation capacity under phosphorus stress does not improve grain yield in green gram[J].Plant Cell Reports, 2014,33(6):919-928.
[16]	White P J.Improving potassium acquisition and utilisation by crop plants[J].Journal of Plant Nutrition and Soil Science,2013,176(3):305-316.
[17]	Vítková M,Komárek M,Tejnecky V,et al.Interactions of nano-oxides with low-molecular-weight organic acids in a contaminated soil[J].Journal of Hazardous Materials,2015, 293:7-14.
[18]	Huang Q,Zhao Z,Chen W.Effects of several low-molecular weight organic acids and phosphate on the adsorption of acid phosphatase by soil colloids and minerals[J]. Chemosphere,2003,52(3):571-579.
[19]	Gunina A,Dippold M A,Glaser B,et al.Fate of low molecular weight organic substances in an arable soil:from microbial uptake to utilisation and stabilization[J].Soil Biology and Biochemistry,2014,77:304-313.
[20]	Yin H,Li Y,Xiao J,et al.Enhanced root exudation stimulates soil nitrogen transformations in a subalpine coniferous forest under experimental warming[J].Global Change Biology, 2013,19(7):2158-2167.
[21]	He Y,Zhan F,Li Y,et al.Effect of enhanced UV-B radiation on methane emission in a paddy field and rice root exudation of low-molecular-weight organic acids[J].Photochemical and Photobiological Sciences,2016,15(6):735-743.
[22]	韦婷婷,杨再强,王琳,等.玻璃温室和塑料大棚内逐时气温模拟模型[J].中国农业气象,2018,39(10):644-655.
	Wei T T,Yang Z Q,Wang L,et al.Simulation model of hourly air temperature inside glass greenhouse and plastic Greenhouse[J].Chinese Journal of Agrometeorology,2018, 39(10):644-655.(in Chinese)
[23]	韦婷婷,杨再强,王明田,等.高温与空气湿度交互对花期番茄植株水分生理的影响[J].中国农业气象,2019,40(5): 317-326.
	Wei T T,Yang Z Q,Wang M T,et al.Effects of high temperature and different air humidity on water physiology of flowering tomato seedlings[J].Chinese Journal of Agrometeorology,2019,40(5):317-326.(in Chinese)
[24]	Yang Z,Li Y,Li P,et al.Effect of difference between day and night temperature on tomato(Lycopersicon esculentum Mill.)root activity and low molecular weight organic acid secretion[J].Soil Science and Plant Nutrition,2016,62(5-6): 423-431.
[25]	李煜姗,李平,杨再强,等.低温寡照影响番茄幼苗根系有机酸代谢和养分吸收[J].中国农业气象,2019,40(8):512-522.
	Li Y S,Li P,Yang Z Q,et al.Low temperature and low irradiation affected the metabolism of low molecular weight organic acids and nutrients uptake in tomato seedling root[J].Chinese Journal of Agrometeorology,2019,40(8): 512-522.(in Chinese)
[26]	Mathur S,Agrawal D,Jajoo A.Photosynthesis:response to high temperature stress[J].Journal of Photochemistry and Photobiology B:Biology,2014,137:116-126.
[27]	Xu C,Yang Z,Wang M,et al.Characteristics and quantitative simulation of stomatal conductance of Panax notoginseng [J].International Journal of Agriculture and Biology,2019, 22(2):388-394.
[28]	Kawasaki Y,Matsuo S,Kanayama Y,et al.Effect of root-zone heating on root growth and activity,nutrient uptake,and fruit yield of tomato at low air temperatures[J].Journal of the Japanese Society for Horticultural Science,2014:MI-001.
[29]	Wu Y,Zhang J,Deng Y,et al.Effects of warming on root diameter,distribution,and longevity in an alpine meadow[J]. Plant Ecology,2014,215(9):1057-1066.
[30]	栾军伟,刘世荣.土壤呼吸的温度敏感性:全球变暖正负反馈的不确定因素[J].生态学报,2012,32(15):4902-4913.
	Luan J W,Liu S R.Temperature sensitivity of soil respiration: uncertainties of global warming positive or negative feedback[J].Acta Ecologica Sinica,2012,32(15):4902-4913. (in Chinese)
[31]	Agnieszka K,Szymon P,Natalia S,et al.A study of low-molecular-weight organic acid urinary profiles in prostate cancer by a new liquid chromatography-tandem mass spectrometry method[J].Journal of Pharmaceutical and Biomedical Analysis,2018,15(10):229-236.
[32]	Galant A L,Kaufman R C,Wilson J D.Glucose:detection and analysis[J].Food Chemistry,2015,188:149-160.
[33]	Mangiapia M,Scott K.From CO2 to cell:energetic expense of creating biomass using the Calvin–Benson–Bassham and reductive citric acid cycles based on genome data[J].FEMS Microbiology Letters,2016,363(7):fnw354.
[34]	吴林坤,林向民,林文雄.根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望[J].植物生态学报,2014,38(3): 298-310.
	Wu L K,Lin X M,Lin W X.Advances and perspective in research on plant-soil-microbe interactions mediated by root exudates[J].Chinese Journal of Plant Ecology,2014,38(3): 298-310.(in Chinese)
[35]	Landi L,Valori F,Ascher J,et al.Root exudate effects on the bacterial communities,CO2 evolution,nitrogen transformations and ATP content of rhizosphere and bulk soils[J].Soil Biology and Biochemistry,2006,38(3):509-516.
[36]	Pan F,Liang Y,Zhang W,et al.Enhanced nitrogen availability in karst ecosystems by oxalic acid release in the rhizosphere [J].Frontiers in Plant Science,2016,7:687.
[37]	Zhang N,Wang D,Liu Y,et al.Effects of different plant root exudates and their organic acid components on chemotaxis, biofilm formation and colonization by beneficial rhizosphere- associated bacterial strains[J].Plant and Soil,2014,374(1-2): 689-700.