Abstract: In order to compare influence of three different cultivation methods(integrated high yield cultivation system-IC,Sanzhuang Sangao cultivation system-SC and Conventional cultivation system-CC) on amino acid content of high protein forage rice grains,field experiments combining with laboratory analysis were conducted in 2004 in Langli County of Changsha City.The rice types provided for the experiments were Xiangfengzao119(early rice) and Peiliangyou288(late rice).and three cultivations methods were high-protein and high-yield integrative cultivation(HPHYIC),san-zhuang-san-gao cultivation(SZSGC) and conventional cultivation(CC).The Glutamicoxalacetic transaminase(GOT) and aspartokinase(AK) activity of leaves and grains,and the contents of protein and amino acid in brown rice were analyzed.The results showed that the activities of GOT and AK in functional leaves and grains,the contents of amino acid,lysine,threonine and methionine,seven kinds of essential amino acids for people,eleven of essential amino acids for pig,fourteen kinds of essential amino acids for chicken,protein and protein yield in brown rice were significantly improved by IC method compared with those of conventional cultivation and SC method.The IC method was suitable for high protein forage rice cultivation.

Key words: Rice, Rice, Glutamicoxalacetic transaminase, Aspartokinase, Amino acid, Protein