Effects of Continuous Overcast Weather on Cucumber Growth and Antioxidant Enzyme Activities in Glasshouse

doi:10.3969/j.issn.1000-6362.2017.09.001

Abstract

Abstract: In order to investigate the effects of overcast weather on cucumber (Cucumis sativus L. cv. Derit L108) growth in glasshouse, the experiment was conducted under two levels of photosynthetic active radiation(PAR): normal irradiation and low irradiation (＜200μmol·m-2·s-1 photon), respectively. Five durations of continuous low irradiation were designed (1, 3, 5, 7 and 9 days), then both groups of plants were transferred under normal irradiation to recover for 9 days. The growth index and the activity of antioxidant enzymes in cucumber leaves were observed. The results showed that: (1) the SPAD value in cucumber leaves increased while the net photosynthesis rate decreased under low irradiation. The longer the duration, the smaller the net photosynthetic rate in cucumber leaves, and the worse on the recovery ability in cucumber leaves. (2) The morphological index of cucumber were significantly affected by low irradiation, and the height, the stem diameter and the leaf-area per plant were smaller under low irradiation after 7 and 9 days than the control respectively, and they could not return to the control level after 9 days under normal irradiation. The cucumber yield and the commercial character decreased with the continuous low irradiation. The activities of SOD, POD were increased and the CAT activity increased at the beginning and then decreased while the content of MDA continuous increased. The longer the duration of low irradiation, the cucumber suffered more by membrane lipid peroxidation. The metabolic function of cucumber leaves was difficult to recover when continuous low irradiation for more than 7 days.

Key words: Overcast weather, Glasshouse, Cucumber, Morphological index, Yield, Enzyme activities

XIONG Yu, DIAO Jia-min, XUE Xiao-ping, LV Xue-mei, ZHANG Ji-bo. Effects of Continuous Overcast Weather on Cucumber Growth and Antioxidant Enzyme Activities in Glasshouse[J]. Chinese Journal of Agrometeorology, 2017, 38(09): 537-547.

References

[1]孔慧英.黄瓜特征特性及无公害栽培技术[J].现代农业科技,2013,(23):94-95. Kong H Y.Characteristics and pollution-free cucumber cultivation techniques of cucumber[J].Modern Agricultural Science and Technology,2013,(23):94-95.(in Chinese) [2]邵慧东.黄瓜特征特性及日光温室栽培技术[J].现代农业科技,2012,(23):92-93. Shao H D.Characteristics and greenhouse cultivation techniques of cucumber[J].Modern Agricultural Science and Technology,2012,(23):92-93.(in Chinese) [3]Guan Y,Chen Q,Pan J S.Construction of a BAC library from cucumber(Cucumis sativus L.) and identification of linkage group specific clones[J].Progress in Natural Science:Materials International,2008,12(2):143-147. [4]刘桂军,赵京岚,薛提燕,等.“春秋先锋5号”水果型黄瓜选育及栽培技术研究[J].北方园艺, 2014,24(18):175-177. Liu G J,Zhao J L,Xue T Y,et al.Study on breeding and cultivation techniques of a new mini fruit cucumber‘Chunqiu Xianfeng No.5'[J].Northern Horticulture,2014,24(18): 175-177. (in Chinese) [5]李春,郭晶.2013-2014年冬季天津地区连续雾霾天气对设施农业生产的影响[J].天津农林科技,2014,(3):36-37. Li C,Guo J.Effect of continuous fog and haze of agricultural production facilities in Tianjin 2013-2014 winter[J].Science and Technology of Tianjin Agriculture and Forestry,2014, (3):36-37.(in Chinese) [6]Lidon F C,Loureiro A S,Vieira D E,et al.Photoinhibition in chilling stressed wheat and maize[J]. Photosynthetica, 2001, 39(2):161-166. [7]毛艺林.雾霾环境对设施农业的影响及应对策略[J].河南农业科学,2014,(7):76-79. Mao Y L.Influence of haze on facility agriculture and coping strategy[J].Journal of Henan Agricultural Sciences,2014, (7): 76-79. [8]Routaboul J M,Fischer S F,Browse J.Trienoic fatty acids are required to maintain chloroplast function at low temperatures[J]. Plant Physiology,2000,124(4):1697-705. [9]Vijayan P,Browse J.Photoinhibition in mutants of Arabidopsis deficient in thylakoid unsaturation[J].Plant Physiology, 2002, 129(2):876-885. [10]李新国,毕玉平,赵世杰,等.短时低温胁迫对甜椒叶绿体超微结构和光系统的影响[J].中国农业科学,2005, 38(6): 1226-1231. Li X G,Bi Y P,Zhao S J,et al.Effects of short-term chilling stress on the photosystems and chloroplast ultrastructure in sweet pepper[J].Scientia Agricultura Sinica,2005,38(6): 1226-1231.(in Chinese) [11]Ody Y.Effects of light intensity,concentration and leaf temperature on gas exchange of strawberry plants-feasibility studies on enrichment in Japanese conditions[J].Acta. Horticultruae, 1997,439:563-573. [12]Shen J R,Terashima I,Katoh S.Cause for dark,chilling- induced inactivation of photosynthetic oxygen-evolving system in cucumber leaves[J].Plant Physiology,1990, 93(93): 1354-1357. [13]Sonoike K.Various aspects of inhibition of photosynthesis under light/chilling stress:“Photoinhibition at chilling temperatures” versus “chilling damage in the light”[J]. Journal of Plant Research,1998,111:121-129. [14]钱芝龙,丁犁平,曹寿椿.低温胁迫对辣(甜)椒膜脂过氧化水平及保护酶活性的影响[J].园艺学报,1994,21(2):203-204. Qian Z L,Ding L P.Effect of temperature stress on pepper seedling lipid peroxidation level and protective enzyme activity[J].Acta Horticulturae Sinica,1994,21(2):203-204.(in Chinese) [15]陈青君,张福漫,王永健,等.临界低温弱光对黄瓜光合特性及其酶变化的影响[J].华北农学报,2003,18(4):31-34. Chen Q J,Zhang F M,Wang Y J,et al.Influence of critical low temperature and poor light on photosynthesis characters and enzyme variance of cucumber[J].Acta Agriculturae Boreali-sinica,2003,18(4):31-34.(in Chinese) [16]艾希珍,马兴庄,于立明,等.弱光下长期亚适温和短期低温对黄瓜生长及光合作用的影响[J].应用生态学报, 2004,15(11):2091-2094. Ai X Z,Ma X Z,Yu L M,et al.Effect of long-term suboptimal temperature and short-term low temperature under low light density on cucumber growth and its photosynthesis[J]. Chinese Journal of Applied Ecology,2004,15(11): 2091-2094. (in Chinese) [17]艾希珍,王秀峰,郭延奎,等.弱光亚适温和低温对黄瓜气孔特性及叶绿体超微结构的影响[J].中国农业科学,2006, 39(10):2063-2068. Ai X Z,Wang X F,Guo Y K,et al.Effects of suboptimal temperature and low temperature under low light intensity on stomatal characteristics and chloroplast ultrastructure of cucumber seedlings[J].Scientia Agricultura Sinica,2006, 39(10):2063-2068.(in Chinese) [18]艾希珍,张振贤,何启伟,等.日光温室主要生态因子变化规律及其对黄瓜光合作用的影响[J].应用与环境生物学报,2002,8(1):41-46. Ai X Z,Zhang Z X,He Q W,et al.Changes in ecological factors and their effects on photosynthesis of cucumber leaves (Cucumis sativus L.) in heliogreenhouse[J].Chinese Journal of Applied & Environmental Biology,2002, 8(1): 41-46.(in Chinese) [19]王绍华,曹卫星,王强盛,等.水稻叶色分布特点与氮素营养诊断[J].中国农业科学,2002,35(12):1461-1466. Wang S H,Cao W X,Wang Q S,et al.Positional distribution of leaf color and diagnosis of nitrogen nutrition in rice plant[J].Scientia Agricultura Sinica,2002,35(12): 1461-1466. (in Chinese) [20]马德华,庞金安.弱光对黄瓜幼苗光合及膜脂过氧化作用的影响[J].河南农业大学学报,1998,23(1):1-3. Ma D H,Pang J A.Effect of low light on photosynthesis and membrane-lipid peroxidation of Cucumis sativus seedling[J].Acta Agriculturae Universitatis Henanensis, 1998, 23(1):1-3.(in Chinese) [21]杨广东,朱祝军.不同光照条件下缺镁对黄瓜生长及活性氧清除系统的影响[J].园艺学报,2001,28(5):430-434. Yang G D,Zhu Z J.Effects of magnesium deficiency on growth and active oxygen scavenging system in cucumber under different light intensities[J].Acta Horticulturae Sinica, 2001,28(5):430-434.(in Chinese) [22]周艳虹,喻景权,钱琼秋,等.低温弱光对黄瓜幼苗生长及抗氧化酶活性的影响[J].应用生态学报,2003,14(6):921-924. Zhou Y H,Yu J Q,Qian Q Q,et al.Effects of chilling and low light on cucumber seedlings growth and their antioxidative enzyme activities[J].Chinese Journal of Applied Ecology, 2003,14(6):921-924.(in Chinese) [23]Yang Z Q,Yuan C H,Han W,et al.Effects of low irradiation on photosynthesis and antioxidant enzyme activities in cucumber during ripening stage[J].Photosynthetic, 2016, 54(2):251-258. [24]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. Li H S.Physiological and biochemical principles and techniques[M].Higher Education Press,2000.(in Chinese) [25]Kumagai E,Araki T,Kubota F.Correlation of chlorophyll meter readings with gas exchange and chlorophyll fluorescence in flag leaves of rice (Oryza sativa L.) plant[J].Plant Production Science,2009,12:50-53. [26]Lin F F,Qiu L F,Deng J S,et al.Investigation of SPAD meter-based indices for estimating rice nitrogen status[J]. Computersand Electronics in Agriculture,2010,71(S):60-65. [27]Green B R,Durnford D G.The chlorophyll-carotenoid proteins of oxygenic photosynthesis[J].Annual Review of Plant Physiology and Plant Molecular Biology, 1996, 47: 685-714. [28]Kornyeyev D,Logan B A,Payton P,et al.Enhanced photochemical light utilization and decreased chilling-in- duced photoinhibition of photosystem II in cotton overex- pressing genes encoding chloroplast-targeted antioxidant enzymes[J].Physiologia Plantarum,2001, 113:323-331 [29]曹锡清.膜质过氧化对细胞与机体的作用[J].生物化学与生物物理进展,1986,(2):17-21. Cao X Q.The role of membrane lipid peroxidation on the cell and body[J].Progress in Biochemistry and Biophysics, 1986,(2):17-21.(in Chinese) [30]冯炘,于贤昌,郭恒俊,等.低温胁迫对黄瓜嫁接苗和自根苗保护酶活性的影响[J].山东农业大学学报:自然科学版,2002,33(3):302-304. Feng X,Yu X C,Guo H J.et al.Effect of low temperature stress on the protective-enzyme activity of grafted cucumber seedling and own-root cucumber seedling[J].Journal of Shandong Agricultural University:Natural Sciences,2002, 33(3):302-304.(in Chinese) [31]Hu W H,Wu Y,Zeng J Z,et al.Chill-induced inhibition of photosynthesis was alleviated by 24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery[J].Photosynthetic,2010,48:537-544. [32]Rossa M M,Oliveira M C,Okamoto O K,et al.Effect of visible light on superoxide dismutase(SOD) activity in the red alga Gracilariopsis tenuifrons(Gracilariales, Rhodophyta) [J].Journal of Applied Phycology,2002,14:151-157.