Effects of Exogenous Trehalose on Growth and Physiological Characteristics of Maize Seedling Roots under Chilling Stress
LIU Xuan, TONG Hao-yang, TIAN Li-xin, ZUO Shi-yu, SUN Lei, QU Dan-yang, LI Li-jie, WEI Shi, LI Jing
2018, 39(08):
538-547.
doi:10.3969/j.issn.1000?6362.2018.08.006
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In order to investigate the trehalose on root growth, antioxidant enzyme activity, cell membrane stability and osmotic adjustment of maize seedlings,"Xianyu335"was chosen as the experimental materials by pot culture method. When the seedlings grow to three leaves, the roots were treated with trehalose at the concentration of 3, 6, 9, 12, 15, 18mmolL?1, with the treatment of spraying clear water as CK. Afterwards, the seedling are placed in artificial intelligence incubator (day/night temperature of 14℃/5℃ low temperature condition) for 4 days. The results showed that the application of trehalose effectively promoted the root growth of maize seedlings under low temperature stress, enhanced the antioxidant enzyme activities, and maintained the stability of cell membrane structure and function of root system. Compared with the control, with the application of 12mmolL?1 trehalose, the root length, root surface area and root dry weight of the seedlings increased by 32.16%, 15.87%, 41.14% and 58.33%, the activities of SOD and POD increased by 44.16% and 62.17%, the content of MDA and the relative conductivity were reduced by 50.23% and 42.53%, and the contents of proline and soluble protein were increased by 81.32% and 87.21%, respectively. The results showed that the optimum concentration of trehalose could significantly improved the low temperature resistance of maize seedlings, alleviated the inhibition of low temperature stress on seedling growth, enhanced the activity of antioxidant enzymes and reduced the membrane damage, and 12mmolL?1 trehalose solution has the best treatment effect.