Analysis the Effect of Different Chemical Agents on High Temperature Stress in Rice Leaves

doi:10.3969/j.issn.1000-6362.2018.02.003

Abstract

Abstract: In this research, early indica hybrid rice Lingliangyou 268 was used for pot experiment. The rice leaves was sprayed with four different concentrations chemical agents, which were 1.5mmol·L-1 and 2.5mmol·L-1 sodium silicate (Na2SiO3·9H2O) solution, 0.5mmol·L-1 and 1.5mmol·L-1 salicylic acid (SA) solution, 10.0mmol·L-1 and 20.0mmol·L-1 calcium chloride(CaCl2·5H2O) solution, 22.04mmol·L-1 and 36.74mmol·L-1 potassium dihydrogen phosphate (KH2PO4) solution respectively, at the jointing stage for 3 days. Five days high temperature stress was conducted in artificial climate box. The daily average temperature of artificial climate box was regulated at 35℃ by setting 40±0.5℃ from 6：00 to 18：00 o’clock, and 30±0.5℃ from 18：00 to 6：00 o’clock of next day. The leaf chlorophyll content, SOD activity, POD activity, CAT activity, MDA and soluble protein content in rice leaves were measured three times, which were 72h and 120h during high temperature treatment and 120h after high temperature treatment finished respectively, to research the alleviative effect of four kinds of chemical agents on high temperature stress in rice. The results showed that the four kinds of chemical agents significantly increased chlorophyll content, SOD activity, POD activity, CAT activity and soluble protein content in rice leaves under high temperature stress with MDA content reducing compared with CK (spraying distilled water). The 20.0mmol·L-1 CaCl2·5H2O and 22.04mmol·L-1 KH2PO4 solutions had the most significant effect. The leaf had the strongest ability to delay senescence under KH2PO4 solution treatment and CaCl2·5H2O solution at 120h of the high temperature treatment and 120h after the high temperature treatment finished.

Key words: High temperature stress, Chemical agent, Rice, Senescence characteristics

JIANG Xiao-dong, JIANG Lin-lin, HUA Meng-fei, CHEN Hui-ling, LV Run, HU Ning, YANG Xiao-ya. Analysis the Effect of Different Chemical Agents on High Temperature Stress in Rice Leaves[J]. Chinese Journal of Agrometeorology, 2018, 39(02): 92-99.

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