Effects of High Temperature Stress and Recovery on Photosynthesis and Stress Tolerance of Tea Leaves

doi:10.3969/j.issn.1000-6362.2016.03.005

Abstract

Abstract:

In order to study the effect of high temperature stress on the photosynthesis rate, fluorescence characteristics, antioxidant enzyme activities and cell injury rates of Camellia sinensis in south region of the Yangtze River, four varieties of tea trees such as Fuding dabaicha, Jiukeng, Longjing 43 and Wuniuzao were investigated under different high temperature stress（35℃ and 40℃,with 25℃ as control）in artificial climate box for 6,12,18,24,48 hours, then recovered for 9 days. The results showed that high temperature stress inhibited the maximum net photosynthetic rate of tea and maximal photochemical efficiency (Fv/Fm) significantly, the maximum net photosynthetic rate and Fv/Fm declined faster when the processing was longer and the temperature was higher. In addition to Fuding dabaicha under 35℃, other varieties of tea could not be recovered to the normal level during a recovery. The SOD activities of Camellia sinensis leaves rose rapidly at the first 12 hours, and then decreased. The average of MDA contents achieved maximum when the temperature were conducted for 48 hours, the leaf cell injury rates increased with high temperature and processing time. The order of the heat resistance among four Camellia sinensis was Fuding dabaicha＞Wuniuza＞Jiukeng＞Longjing 43. The study provided a basis for scientific research of Camellia sinensis.

Key words: High temperature stress, Photosynthetic characteristics, Antioxidant enzyme activity, Cell injury rates

HAN Dong，YANG Fei，YANG Zai-qiang，JIN Zhi-feng. Effects of High Temperature Stress and Recovery on Photosynthesis and Stress Tolerance of Tea Leaves[J]. Chinese Journal of Agrometeorology, 2016, 37(03): 297-306.

References

[1]张玉翠,王连翠.低温对茶树叶片膜透性和保护酶活性的影响[J].北方园艺,2010,(9):38-40.

Zhang Y C,Wang L C.Effect of low temperature stress on membrane permeability and protection of activity in tea leaves[J].Northern Horticulture,2010,(9):38-40.(in Chinese)

[2]李浣沧.浙江优质绿茶的气象条件分析[J].茶叶,1982, (3):1-4.

LI H C.An analysis of meteorological conditions of Zhejiang high quality green tea[J].Journal of Tea,1982, (3):1-4.(in Chinese)

[3]Karim M A,Fracheboud Y,Stamp P.Photosynthetic activity of developing leaves of Zea Mays is less affected by heat stress than that of developed leaves[J].Physiology Plant,1999,105: 685-693.

[4]孟令波,秦智伟,李淑敏.高温胁迫对黄瓜幼苗根系生长的影响[J].园艺学报,2003,30(6):694-695.

Meng L B,Qin Z W,Li S M.Effects of high temperature stress on the root of cucumber seeding[J].Acta Horticulturae Sinica,2003,30(6):694-695.(in Chinese)

[5]Howell G S.Sustainable grape productivity and the growth-yield relationship:a review[J].American Journal of Enology and Viticulture,2001,52:165-174.

[6]Caprio J M,Quamme H A.Weather conditions associated with grape production in the Okanagan Valley of British Columbia and potential impact of climate change[J].Canadian Journal of Plant Science,2002,82: 755-763.

[7]王冬梅,许向阳,李景富.番茄耐热性研究进展[J].中国蔬菜, 2003,(2):58-60.

Wang D M,Xu X Y,Li J F.Advances of heat-resistant in tomato[J].China Vegetables,2003,(2):58-60.(in Chinese)

[8]刘爱荣,陈双臣,王森博,等.高温胁迫对番茄幼苗光合作用和叶绿素荧光参数的影响[J].西北农业学报, 2010,19(5): 145-148.

Liu A R,Chen S C,Wang S B,et al.Effects of heat stress on photosynthesis and chlorophyll fluorescence parameters in tomato seedlings[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(5):145-148.(in Chinese)

[9]Farquar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annu Rev Plant Physiol,1982,(33):317-345.

[10]孙宪芝,郑成淑,王秀峰.高温胁迫对切花菊-神马光合作用与叶绿素荧光的影响[J].应用生态学报,2008,19(10): 214-215.

Sun X Z,Zheng C S,Wang X F.Effects of high temperature stress on photosynthesis and chlorophyll fluorescence of cut flower chrysanthemum(Dendranthema grandif lora-Jinba.) [J]. Chinese Journal of Applied Ecology,2008,19(10): 214-215.(in Chinese)

[11]吴韩英,寿森炎,朱祝军.高温胁迫对甜椒的光合作用和叶绿素荧光的影响[J].园艺学报,2001,28(6): 517-521.

Wu H Y,Shou S Y,Zhu Z J.Effects of high temperature stress on photosynthesis and chlorophyll fluorescence in sweet pepper(Capsicum fructescens L)[J].Acta Horticulturae Sinica,2001,28(6):517-521.(in Chinese)

[12]Lu P,Sang W G,Ma K P.Differential responses of the activities of antioxidant enzymes to thermal stresses between two invasive Eupatorium species in China[J].Journal of Integrative Plant Biology,2008,50(4): 393-401.

[13]Shin Y,Ryu J A,Liu R H,et al.Harvest maturity, storage temperature and relative humidity affect fruit quality,antioxidant contents and activity,and inhibition of cell proliferation of strawberry fruit[J].Postharvest Biology and Technology,2008,49:201-209.

[14]Jiang Y W,Huang B R.Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation[J].Crop Science,2001,41(2):436-442.

[15]Zhao X,Nishimura Y,Fukumoto,et al.Effect of high temperature on active oxygen species,senescence and photosynthetic in cucumber leaves[J].Environmental and Experimental Botany,2011,70:212-216.

[16]Martineau J R,Specht J E.Temperature tolerance in soy beans[J].Crop Science,1979,(19):75-81.

[17]Martina S,Wolfgang M,Cosima W,et al.Ozone-induced oxidative burst in the ozone bicmonitor plant tobacco BelW_s[J].The Plant J,1998,(16):235-245.

[18]李建建,郁继华,常雅君,等.高温胁迫对黄瓜幼苗叶片质膜透性及保护酶活性的影响[J].长江蔬菜,2007,(9):59-61.

Li J J,Yu J H,Chang Y J,et al.Influences of high temperature stress on membrane permeability and activity of cell defence enzymes in leaves of cucumber seedlings[J].Journal of Changjiang Vegetables,2007,(9):59-61. (in Chinese)

[19]张桂莲,廖斌,汤平,等.灌浆结实期高温对水稻剑叶生理特性和稻米品质的影响[J].中国农业气象,2014,35(6):650- 655.

Zhang G L,Liao B,Tang P,et al.Effects of high temperature stress during grain-filling period on physiological characteristics in flag leaves and grain quality of rice[J]. Chinese Journal of Agrometeorology,2014, 35(6):650-655. (in Chinese)

[20]钟芳永.不同肥料处理对茶树生理特性及茶叶品质的影响[D].福州:福建农林大学,2013.

Zhong F Y.Effect of tea plant physiological characteristics and quality under different fertilizer treatments[D]. Fuzhou: Fujian Agriculture and Forestry University,2013.(in Chinese)

[21]赵甜甜,蔡新.不同遮荫度下茶树生理生化特性的研究[J].湖南农业科学,2010,(5):38-41.

Zhao T T,Cai X.Physiological and biochemical characters of tea-plants with different shading degrees[J]. Hunan Agricultural Sciences,2010,(5):38-41.(in Chinese)

[22]肖润林,王久荣,汤宇,等.高温干旱季节遮阳网覆盖对茶园温湿度和茶树生理的影响[J].生态学杂志, 2005,24(3): 251-255.

Xiao R L,Wang J R,Tang Y,et al.Effects of covering with outer shading screens during hot-dry season in tea plantation[J].Chinese Journal o f Ecology,2005,24(3):251- 255.(in Chinese)

[23]Chance B,Maehly A C.Assay of catalase and peroxidase: methods of enzymology[J].New York:Academic Press,1955: 764-755.

[24]Rabinowitch H D,Sklan D.Sunscald tolerance in tomatoes: role of superoxide dismutase[J].Planta,1980, 148 (2):162- 167.

[25]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2001:258-260.

Li H S.Principles and techniques of plant physiological biochemical experiment[M].Beijing:Higher Education Press, 2001:258-260.(in Chinese)

[26]Bradford M M.A rapid and sensitive method for the determination of microgram quantities of protein utilizing the principle of protein-dyebinding[J].Analytical Biochemistry, 1976,72:248-255.

[27]刘建锋,杨文娟,江泽平,等.遮荫对濒危植物崖柏光合作用和叶绿素荧光参数的影响[J].生态学报,2011, 31(20): 5999-6004.

Liu J F,Yang W J,Jiang Z P,et al.Effects of shading on photosynthetic characteristics and chlorophyⅡ fluorescence parameters in leaves of the endangered plant Thuja sutchuenensis[J].Acta Ecologica Sinica,2011,31(20): 5999-6004.(in Chinese)

[28]许大全,张玉忠,张荣铣.植物光合作用的光抑制[J].植物生理学通讯,1992,28(4):237-243.

Xu D Q,Zhang Y Z,Zhang R X.Photoinhibition of photosynthesis in plants[J].Plant Physiology Communica- tions,1992,28(4): 237-243.(in Chinese)

[29]Takakura T.Dynamic simulation of plant growth and environment in the greenhouse[J].Transaction of ASAE,1969, 14(5):964-971.

[30]李萍萍,胡永光,赵玉国.叶用莴苣温室栽培单株光合作用日变化规律[J].园艺学报,2001,28(3):240-245.

Li P P,Hu Y G,Zhao Y G.A study on daily variation of photosynthesis in greenhouse butterhead lettuce[J]. Acta Horticulturae Sinica,2001,28(3):240-245.(in Chinese)

[31]刘玉华,史纪安,贾志宽.旱作条件下紫花苜蓿光合蒸腾日变化与环境因子的关系[J].应用生态学报,2006, 17(10): 231-238.

Liu Y H,Shi J A,Jia Z K.Diurnal dynamics of photosynthetic and transpiration rates of alfalfa under dry farming and their relationships with environmental factors[J].Chinese Journal of Applied Ecology,2006,17(10):231-238.(in Chinese)

[32]谢晓金,李秉柏,朱红霞,等.抽穗期高温对水稻叶片光合特性和干物质积累的影响[J].中国农业气象, 2012,33(3): 457-461.

Xie X J,Li B B,Zhu H X,et al.Impact of high temperature at heading stage on rice photosynthetic characteristic and dry matter accumulation[J].Chinese Journal of Agrometeorology, 2012,33(3):457-461.(in Chinese)

[33]罗海波,马菩,段伟,等.高温胁迫对赤霞珠葡萄光合作用的影响[J].中国农业科学,2010,43(13):2744-2750.

Luo H B,Ma P,Duan W,et al.Influence of heat stress on photosynthesis in Vitis vinifera L cv Cabernet sauvignon [J].Scientia Agricultura Sinica,2010,43(13):2744-2750.(in Chinese)

[34]金春燕.高温胁迫对番茄幼苗生长及生理代谢的影响[D]. 南京:南京农业大学,2011.

Jin C Y.Study on growth and physiological metabolism of tomato seedlings under high temperature stress[D]. Nanjing: Nanjing Agricultural University,2011.(in Chinese)

[35]杨再强,李伶俐,殷剑敏,等.灌浆初期不同时长高温胁迫对早稻叶片光合和荧光参数的影响[J].中国农业气象,2014, 35(1):80-84.

Yang Z Q,Li L L,Yin J M,et al. Effects of different duration of high temperature stress during filling stages on leaves photosynthetic and fluorescence parameters of early rice[J].Chinese Journal of Agrometeorology, 2014,35(1): 80-84.(in Chinese)

[36]马旭俊,朱大海.植物超氧化酶(SOD)的研究进展[J].遗传,2003,25(2):225-231.

Ma J X,Zhu D H.Functional roles of the plant superoxide dismutase[J].Hereditas,2003,25(2):225-231.(in Chinese)

[37]李天来,李森,孙周平.钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用[J].应用生态学报,2009,20(3): 556-590.

Li T L,Li S,Sun Z P.Regulation effect of calcium and salicylicacid on defense enzyme activities in tomato leaves under sub-high temperature stress[J].Chinese Journal of Applied Ecology,2009,20(3):556-590.(in Chinese)

[38]何跃君,薛立,任向荣,等.低温胁迫对六种苗木生理特性的影响[J].生态学杂志,2008,27(4):524-531.

He Y J,Xue L,Ren X R,et al.Effects of low temperature stress on physiological characteristics of six tree spec ies seedlings[J]. Chinese Journal o f Ecology,2008,27(4):524-531.(in Chinese)

[39]黄海涛,余继忠,张伟,等.电导法配合Logistic方程鉴定茶树抗寒性的探讨[J].浙江农业科学,2009,(3):577-579.

Huang H T,YU J Z,Zhang W,et al.Discussion on measurement of cold tolerance based on REC and the Logistic equation in tea tree[J].Journal of Zhejiang Agricultural Science,2009,(3): 577-579.(in Chinese)

[40]Tsarouhas V,Kenney W A,Zsuffa L.Application of two electrical methods for the rapid assessment of freezing resistance in Salix eriocephala[J].Biomass and Bioenergy, 2000,19(3): 165-175.