A field experiment was conducted to investigate the effects of exogenous salicylic acid on diurnal variations of the net photosynthetic rate (Pn), transpiration rate (Tr), stomata conductance (Gs), intercellular CO2 concentration(Ci), and leaf water use efficiency (WUE) of peanuts leaves at acicula forming stage under the enhanced UV-B with a portable photosynthesis system (LI-6400, USA). Two plots, i.e., the natural light plot and UV-B radiation enhancement plot (E, increase the amount equivalent to local 4-5 months), was set, both with two small plots be sprayed respectively with distilled water (S0) and salicylic acid (SA) for 3 days in a fixed period from the acicula forming stage (July 25). The results showed that, compared with CK, the Pn, Tr, Gs, and WUE of peanuts leaves under S0 treatment with 20% enhanced UV-B radiation decreased 35.7%, 25.0%, 25.0% and 10.0%, and then those of SA treatment decreased 30.4%, 17.9%, 35.3% and 19.4%, respectively. The results indicated that the net photosynthetic rate, transpiration rate, stomata conductance, and water use efficiency of peanuts leaves could be reduced under enhanced UV-B radiation, and the salicylic acid could relieve the inhibitory effect of UV-B radiation on net photosynthetic rate. However, it could not relieve the inhibitory effect of UV-B radiation on the transpiration rate, stomata conductance, and water use efficiency of peanuts leaves.